中文摘要：

以薏苡（Coixchinensis）种子为实验材料，通过脱脂、水提、ResourceS阳离子交换层析和Superdex75凝胶排15H层析等步骤纯化得到一种新的抗真菌蛋白，并对其抑菌活性进行初步攀定。SDS-PAGE分析显示该蛋白的分子质量约为28kD。抑菌活性鉴定表明，该蛋白对链格孢霉似lternariaalternate）、绿色木霉（Trichodermareesei）和白腐菌（Panusconchatus）3株丝状真菌具有显著的生长抑制活性，并呈现出剂量依赖的特征。本研究建立分离薏苡28kD抗真菌蛋白的方法，制备方法简单易行，所得产品纯度达到95％以上。

英文摘要：

A protein from adlay （Coix chinensis） seeds was isolated by degrease with acetone and ether, extraction with 20 mmol/L Tris-HC1 buffer, ion exchange chromatography on Resource S column and size exclusion chromatography on Superdex 75 column. The purity was higher than 95%. Partial characterization and antifungal activity were assayed. The protein exhibited a molecule mass of 28 kD in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This protein could exert potent inhibi- tory activity toward funga/species including Alternaria alternate, Trichoderma reesei and Panus conchatus in a dose-dependent pattern. Antifungal protein has become a popular research topic because of their resistance to pathogenic diseases. In this paper, the studies have demonstrated that adlay seeds have potentially exploitable significance and explored an effective method for the isolation and purification of antifungal proteins from plant seeds.