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中文摘要：
从苦荞中提取制备异槲皮苷,研究其对人胃癌细胞SGC-7901增殖、凋亡、迁移和细胞周期的影响。将异槲皮苷作用于人胃癌SGC-7901细胞和人肾上皮细胞系293T,通过噻唑蓝法检测异槲皮苷对其增殖的影响;4’,6-二脒基-2-苯基吲哚（4’,6-diamino-2-phenyl indole,DAPI）荧光染色法观察细胞核的形态学变化;划痕擦伤迁移实验检测异槲皮苷对SGC-7901细胞迁移能力的影响;流式细胞术检测异槲皮苷对SGC-7901细胞凋亡及细胞周期的影响。结果表明：苦荞异槲皮苷可以抑制SGC-7901细胞的增殖,并呈时间和剂量依赖性,当用100μmol/L异槲皮苷作用细胞48 h后,对SGC-7901细胞的增殖抑制率达到35.92%,而对人肾上皮细胞系293T的增殖抑制率仅为3.15%;DAPI荧光染色法观察异槲皮苷处理细胞后,染色体凝聚,有凋亡小体产生;划痕擦伤实验显示,异槲皮苷能抑制SGC-7901细胞的迁移;流式细胞术检测结果表明,异槲皮苷可使G1和S期细胞减少,G2/M期细胞增多,且细胞凋亡率明显增加。综上所述,苦荞麦异槲皮苷能够诱导SGC-7901细胞发生凋亡,阻断细胞周期并抑制细胞增殖和迁移。
英文摘要：
Isoquercetin was extracted from Fagopyrum tataricum and investigated for its effect on cell proliferation, apoptosis and migration of human gastric carcinoma cell line SGC-7901. Cell proliferation was examined by using MTT assay. The morphological changes of SGC-7901 were observed by DAPI nuclear staining. The effect of Fagopyrum tataricum isoquercetin on cell migration was investigated by scratch-recovering tests in cell culture plates, as well as its effect on cell apoptosis by flow cytometry. As a result, MTT assay showed that the isoquercetin could inhibit the viability of SGC-7901 cells in time- and dose-dependent manners. SGC-7901 cell growth was inhibited by 35.92% or more at isoquercetin concentration of 100 μmol/L for 48 h, compared to only 3.15% for 293T cells under the same conditions of concentration and culture duration. DAPI nuclear staining showed chromosomal condensation and the production of apoptotic bodies. Scratch-recovering tests indicated that the isoquercetin could suppress cell migration. Flow cytometry showed that the number of cells at the G 1 phase and S phase decreased and increased at the G 2 /M phase and the apoptotic cell population exhibited a signif icant increase. Taken together, isoquercetin from F. tataricum can induce apoptosis, arrest cell cycle and inhibit cell proliferation and migration of SGC-7901 cells.
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