中文摘要：

为了提高离子液体共溶剂效应强化选择性酶促合成异槲皮苷的得率,在前期单因素实验的基础上,利用响应曲面法对离子液体比例、酶浓度和底物浓度三因素进行优化。优化后的最佳工艺条件为：离子液体比例13.05%、酶浓度9.10 g/L、底物浓度0.72 g/L,优化后异槲皮苷的摩尔得率为99.27 ± 0.55%,较优化前提高了8.60%。

英文摘要：

To improve the yield of enzymatic synthesis of isoquercitrin, the process condition of enzymatic reaction was optimized by response surface methodology. According to the results of single-factors experiments, response surface methodology was investigated the selected three factors： ionic liquid content, enzyme concentration and substrate concentration. The result showed that the optimum condition for the enzymatic synthesis of isoquercitrin using ionic liquid as a co-solvent was： ionic liquid content 13.05%, enzyme concentration 9.10 g/L, substrate concentration 0.72 g/L. Under the optimal condition, the yield of isoquercitrin was 99.27 ？ 0.55%, increased by 8.60% than before.