中文摘要：

目的探讨波形蛋白瓜氨酸化前后在体外对类风湿关节炎（RA）患者外周血来源树突状细胞（DCs）的细胞形态、表型和功能的影响。方法分离外周血单个核细胞（PBMCs），粒细胞一巨噬细胞集落刺激因子（GM—CSF）和白细胞介素（IL）-4体外培养制备未成熟DCs（imDCs），分别用脂多糖、瓜氨酸化波形蛋白（cVim）和波形蛋白刺激培养的imDCs，磷酸盐缓冲液（PBS）作为阴性对照，流式细胞仪检测DCs表面标志CD14、CD80、CD83、CD86、主要组织相容性复合体（MHC）Ⅰ、MHCⅡ表达变化。将实验组获得的DCs和同种异体T细胞进行混合淋巴细胞反应，通过单溶液细胞增殖分析（MTS）法检测T细胞的增殖情况。采用t检验。结果以阴性对照组imDCs的各个免疫表型阳性率作为1，脂多糖能显著诱导RA—imDCs表面MHCII、CD80、CD83、CD86表达（1．07±0．14，1．25±0．13，1．90±1．08，2．44±0．65，P均〈O．05）；cVim诱导MHCⅡ（1．18±0．09）、CDS3（1．97±0．99）表达水平上调（P〈0．05，P〈0．01）；波形蛋白对MHCⅡ及CD83、CD86表达无影响（0．95±0．11，0．90±0．29，1．04±0．06），仅抑制CD80表达（0．82±0．18，P〈0．01）。与脂多糖组相比，cVim组的MHCII表达水平显著增高（P〈O．05），而CDS0、CD86（0．83±0．36、1．32±0．15）的表达水平则低于脂多糖组（P〈0．01，P〈0．05）；eVim组MHCⅡ和CD83的表达水平显著高于波形蛋白组（P均〈O．05）。混合淋巴细胞反应显示经脂多糖和cVim诱生的DCs对同种异体T细胞具有明显刺激增殖作用，且随着DCs细胞浓度的增加而作用增强；波形蛋白诱生的DCs则无刺激增殖作用。结论cVim可诱导imDCs成熟，并且能够提高细胞表面共刺激分子的表达。

英文摘要：

Objective To study the effects of citrullinated vimentin （cVim） on the maturation and immunologic function of dendritic cells （DCs） from rheumatoid arthritis （RA） peripheral blood. Methods In the present study, mononuclear cells were isolated from the peripheral blood of patients with RA and cultivated in media containing GM-CSF and IL-4 to generate immature DCs （imDCs）. The imDCs generated were stimulated with citrullinated vimentin and vimentin. LPS was used as the positive control and PBS was used as the negative control. The expression of surface molecules on the DCs, such as CD14, CDS0, CD83, CD86, MHC I and MHC II were analyzed with FACS. The capability of the stimulatory activity of the DCs on allogeneic T cells in mixed reaction was tested by MTS. t-test was used for statistical analysis. Results Compared to untreated DCs, DCs treated with LPS increased the expression levels of MHC Ⅱ , CDS0, CD83 and CD86 （1.07±0.14, 1.25±0.13, 1.90±1.08, 2.44±0.65, P〈0.05）, while cVim increased the expression levels of MHCII （1.18±0.09, P〈0.05） and CD83 （1;97±0.99, P〈0.01）, and Vim decreased the expression levels of CD80 （0.82±0.18, P〈0.01）. It was demonstrated that the expression levels of MHC II on DCs pulsed with cVim were significantly higher than that of the DCs with LPS, but the expression levels of CD80 and CD86 were not significantly different. The. expression levels of MHC It and CD83 on DCs pulsed with cVim were significantly higher than that of the DCs with Vim. The mixed lymphocyte reaction showed that the DCsinduced by IX＇S and cVim trigerred the proli-feration of allogenic T cells obviously. Conclusion This result suggests that cVim could promote the phenotypic maturation of DCs and increase the expression of costimulatory molecules.