中文摘要：

目的观察miR-124对内皮细胞增殖和凋亡的影响,探讨miR-124在动脉粥样硬化中的作用。方法采用荧光定量PCR检测miR-124在40例冠心病患者及40例正常对照血浆中的表达水平,CCK-8和BrdU法检测miR-124对人脐静脉内皮细胞（HUVECs）增殖的影响,TUNNEL染色检测miR-124对HUVECs凋亡的影响,Western blotting检测miR-124对HUVECs促凋亡蛋白cleaved caspase 3及抗凋亡蛋白Bcl-2表达的影响。结果与对照组比较,miR-124在冠心病患者血浆中的表达明显降低。CCK-8和BrdU检测结果提示miR-124可明显促进HUVECs增殖;TUNNEL染色及Western blotting检测结果提示,miR-124可明显降低HUVECs凋亡细胞的比例及cleaved caspase 3表达,同时增加Bcl-2的表达。结论Mi R-124可促进HUVECs的增殖并抑制其凋亡,为深入认识动脉粥样硬化的发病机制提供了理论依据。

英文摘要：

Objective To observe the effects of miR-124 on the proliferation and apoptosis of endothelial cells of umbilical cord vein in order to explore the role of miR-124 in the pathogenesis of atherosclerosis. Methods The expression levels of miR-124 in plasma of 40 patients with coronary heart disease and 40 healthy control participants were examined using quantitative RTPCR. CCK8 assay and BrdU incorporation assay were used to analyze the effects of miR-124 on the proliferation of human umbilical vein endothelial cells（HUVECs）. The effect of miR-124 on HUVEC apoptosis was examined by TUNNEL staining. The effects of miR-124 on the expression of proapoptotic protein cleaved caspase 3 and anti-apoptotic protein Bcl-2 in HUVECs were examined by Western blotting. Results Compared with healthy controls, plasma miR-124 was down-regulated in patients with coronary artery disease. In vitro experiment, miR-124 significantly promoted HUVEC proliferation, and it also inhibited HUVECs apoptosis by affecting the expressions of cleaved caspase 3 and Bcl-2. Conclusions Mi R-124 could promote the proliferation and inhibit the apoptosis of HUVECs. This may provide a theoretical basis for clarifying the mechanisms of atherosclerosis.