中文摘要：

目的：探讨黄连素（Berberine,BBR）在小鼠心肌梗死（myocardial infarction,MI）后心室重构中的作用,并比较BBR预处理（BBR pre-treatment,preBBR）和BBR后处理（BBR post-treatment,postBBR）给药的效果。方法：将60只C57BL/6小鼠随机分为4组,分别为假手术组、单纯MI对照组、MI＋preBBR组及MI＋postBBR组,每组15只。MI模型采用前降支结扎法制备。MI＋preBBR组在MI模型制备前2周开始用BBR（100 mg·kg~（-1）·d~（-1））每天灌胃,持续至MI后28 d;MI＋postBBR组在MI模型制备后4 h开始用BBR（100 mg·kg~（-1）·d~（-1））每天灌胃,持续至MI后28 d。记录实验期间小鼠生存情况。MI后28天采用小动物超声测定左心室收缩功能;取心脏组织,测定心脏大小和重量;ELISA方法测定血浆BNP水平;Masson染色评价心肌纤维化程度。结果：与单纯MI对照组相比,MI＋preBBR组及MI＋postBBR组小鼠生存率提高、心脏收缩功能增强、心脏变小、心脏重量减轻、血浆BNP水平降低、心肌纤维化明显改善。其中,MI＋preBBR组上述指标的改善程度优于MI＋postBBR组。结论：BBR可抑制MI后心室重构,且BBR预处理效果优于后处理。

英文摘要：

Objective： To investigate the role of berberine（BBR） in the ventricular remodeling after myocardial infarction（MI） in mice, and compare the effect of BBR pre-treatment（preBBR） and BBR post-treatment（post-BBR）. Methods： A total of 60 C57BL/6mice were randomly divided into 4 groups, which were Sham group, MI control group, MI＋preBBR group and MI＋postBBR group（n=15 in each group）. MI model was established by ligation of left anterior descending coronary artery. In MI＋preBBR group, BBR（100mg·kg~（-1）·d~（-1）） was given by gavage once a day from 2 weeks prior-MI until 28 days post-MI; while in MI＋postBBR group, BBR（100 mg kg~（-1）·d~（-1）） was given once a day from 4 hours post-MI to 28 days post-MI. Murine survival were recorded daily. Twenty-eight days after MI, left ventricular systolic function was assessed by small animal echocardiography. Size and weight of collected hearts were measured.Plasma BNP level was quantified by ELISA. Cardiac fibrosis was determined by Masson＇s staining. Results： Compared to MI control group, both pre-BBR and post-BBR showed increased survival rate, improved systolic function, reduced heart size and weight, alleviated serum BNP level and cardiac fibrosis. Between MI＋preBBR group and MI＋postBBR group, the former had better results than the latter.Conclusion： BBR inhibited post-MI ventricular remodeling in mice, and therapeutic effect of BBR pretreatment was better than that of post-treatment.