中文摘要：

利用简并PCR克隆技术,扩增和克隆了大鳞副泥鳅（Paramisgurnus dabryanus）Dmrt基因的DM结构域,获得了3个具有不同DM序列的克隆。结果表明,在大鳞副泥鳅基因组中存在Dmrt基因家族的多个成员。同源性比较和系统进化分析显示不同进化地位脊椎动物的Dmrt基因存在高度的进化保守性。

英文摘要：

We amplified genomic DNA of the Paramisgurnus dabryanus using the DM degenerate primers and detected a band, approximately 150 bp. After cloned into pMD18-T vector and sequenced, three sequences showed high homology with the DM domain. They were named as PdDmrtl, PdDmrt3, and PdDmrt5. Based on similarities of amino acid sequences of the DM domain, Dmrt gene protein sequences from six vertebrates species were included in a phylogenetic tree. It confirmed the identity of the three PCR products. Our results reveal that Dmrt gene is highly conservative in phylogeny.