以木薯品种SC124为试材,采用RT-PCR的方法,克隆了木薯可溶性淀粉合成酶基因SSII的全长,并对其进行生物学分析。结果表明:克隆的基因全长包含完整的开放阅读框2256bp,编码751个氨基酸,通过核苷酸序列同源性分析,该cDNA与基因库中登录的木薯SSII(登录号为:EF667961.1)序列同源性达到99%,但与其它植物的同源性较低,为77%~84Yd。对木薯SSII多态性分析可知,在木薯基因组内存在等位基因及旁系同源基因。
Taking Manihot esculenta Crantz cultivar SC124 as material,by RT-PCR technology, the whole sequeee of SSII gene was firstly obtained. The results showed that the gene contained an 2 256 bp open reading frame (ORF) of SSII and encoding 751 amino acids. Comparaing to the published sequence(EF667961. 1), the homology of nucleotide sequence could shares 99 %. However,the homology was low comaraing with other plants,about 77%-84%. The analysis of SSII polymorphism showed that there were alleles and paralogous gene in Manihot esculenta Crantz gene groups.