中文摘要：

目的：研究隐孔菌多糖（CP）对脂多糖（LPS）诱导人肺上皮细胞株A549生成单核细胞趋化蛋白-1（MCP-1）的影响。方法：在有或无CP条件下用LPS诱导A549细胞株，分别采用ELISA和RTPCR法，测定MCP-1蛋白浓度和MCP-1 mRNA的表达。结果：LPS 1000μg／L诱导A549细胞24h明显增加MCP1的生成。CP 100μg／L或地塞米松1μmol／L对A549细胞株的生长和活力无明显影响。CP100μg／L或地塞米松1μmol／L能明显抑制LPS诱导A549细胞株的MCP-1蛋白含量和mRNA的表达。结论：结果证明，CP能调节MCP-1的生成，可能是其抗肺部炎症的作用机制之一。

英文摘要：

Objective： To investigate the effect of cryptoporus polysaccharide （CP） on lipopolysaccharide （LPS）-induced production of monocyte chemoattractant protein-1 （MCP 1）in human lung epithelial A549 cells. Methods： A549 cells were stimulated with LPS in the presence or absence of CP. The protein concentration and mRNA expression of MCP-1 were determined by enzyme-linked immunosobent assay （ELISA） and semi-quantitative RT-PCR, respectively. Results： The protein concentration of MCP-1 was significantly increased by LPS 1000 μg/L at 24 h. There were no effects on the growth and viability of A549 cells in the presence of CP 100 μg/ L or dexamethasone 1 μmol/L. However, CP 100 μg/L or dexamethasone 1μmol/L significantly inhibited the protein concentration and mRNA expression of MCP-1 induced by LPS. Conclusion： CP can regulate MCP-1 production, which may be associated with its effects on lung inflammation.