中文摘要：

[目的]利用基因芯片重注释寻找肝细胞肝癌（hepatocellular carcinoma,HCC）术后复发相关lncRNA（long non-codingRNA）。[方法]首先利用生物信息的方式重新注释35/41例复发/未复发HCC病例的基因表达谱数据,筛查与HCC术后复发相关的差异表达lncRNA。然后利用ROC曲线评价差异lncRNA的术后复发预测效能。最后利用基因共表达网络与功能富集分析探究差异lncRNA具体的生物学功能。[结果]差异表达分析发现3个lncRNA（LINC00342,LINC00607,LINC01553）显著差异表达[｜log（fold change）｜〉1,q value〈0.05]。ROC曲线分析发现该3个差异lncRNA的AUC值为0.693。基因共表达网络分析发现LINC00342相关共表达网络显著富集到＂细胞周期＂相关功能;LINC00607相关的共表达网络中基因主要富集到＂炎症反应＂与＂免疫反应＂相关的生物学功能与通路;LINC01553相关的共表达网络中基因主要富集到＂DNA复制与修复＂相关的生物学功能与通路。[结论]3个lncRNA与HCC术后复发显著相关,可作为一种潜在的诊断标志物组合,值得进一步的挖掘与研究。

英文摘要：

[Objective] To identify the lncRNAs related to postoperative recurrence of hepatocellular carcinoma（HCC） by microarray re-annotation strategy. [Methods] The differentially expressed lncRNAs were identified in 35 cases of HCC with postoperative recurrence and 41 cases of HCC without recurrence by lncRNA re-annotation strategy. The classification power of the lncRNAs was analyzed by ROC curve. The biological functions and pathway related to these lncRNAs were investigated by gene co-expression network and gene functional enrichment analysis. [Results] Three differentially expressed lncRNAs（LINC00342,LINC00607,LINC01553） were found in the dataset [（log（fold change） 〈1,q value〈0.05）]. The AUC score of the three lncRNAs was 0.693 by ROC curve analysis. Co-expression network analysis and gene functional enrichment analysis showed that the genes in LINC00342 related co-expression network were enriched in cell cycle related biological functions;genes in LINC00607 related co-expression network were enriched in inflammatory response and immune response related biological functions;genes in LINC01553 related co-expression network were enriched in DNA replication and repair related biological functions. [Conclusion] The lncRNAs identified in the study may be involved in postoperative recurrence of HCC,which might be a potential biomarker for HCC prognosis and worth of further investigation.