中文摘要：

目的研究甲胎蛋白（Afp）阳性细胞的增殖分化在组织生长修复，肿瘤发生过程中的作用，建立Afp．CreERT转基因小鼠细胞示踪系统。方法采用DNA雄原核显微注射方法获得Afp—CreERT转基因小鼠，筛选出合适的品系后，使之与Rosa26-lacZ工具小鼠杂交，获得Cre/acZ双阳性的Afp—cre—lacZ转基因小鼠。结果显微注射后，共出生小鼠56只，经PCR鉴定Cre阳性小鼠共4只，阳性小鼠传代后各为一系。筛选内源性Alp表达与Cre表达相对符合的品系作为Afp．CreERT转基因小鼠品系建系。Afp．CreERT转基因小鼠与Rosa26-lacZ工具小鼠杂交，经PCR鉴定后获得CreflacZ双阳性的Afp—cre—lacZ转基因小鼠。经实时PCR，X—gal染色，免疫荧光染色鉴定后得到Afp—cre—lacZ转基因小鼠细胞示踪系统，同时证实该系统能够正确示踪Afp表达阳性的细胞，同时也能够应用于肝损伤小鼠模型中。结论成功构建了Afp-cre-lacZ转基因小鼠细胞示踪系统，为研究这类细胞的谱系发生提供了工具。

英文摘要：

Objective To study the function of alpha-fetoprotein （Afp） positive cells during tissues growth and repair or in tumorigenesis, Afp-cre-lacZ linage tracing mouse model was established. Methods Afp-CreERT mice were established by microinjection and identified by real time PCR. Afp- CreERT mice were crossed with Rosa26-1acZ mice. Cre/lacZ both positive mice were kept as Afp-crelacZ mice. Results Fifty-six transgenic mice were born after microinjection. Four of them genotyped Cre positive were kept as founder. Each founder was established a strain. Cre expression of each strain was identified via real time PCR. Thus Afp-CreERT transgenic mouse was established. The Cre/lacZ both positive offspring of Afp-CreERT mice were crossed with Rosa26-1acZ mice were kept as Afp-cre- lacZ mice. Identified by real time PCR, X-gal staining and immunofluorescence, Afp-cre-lacZ mice were observed that they could be used in lineage tracing and further studies. Conclusions The Afp-cre-lacZ mice established may be used as a tool for lineage tracing studies.