中文摘要：

目的采用LC-MS/MS法同时测定养血清脑颗粒（当归、川芎、白芍等）中芍药苷、芍药内酯苷、新绿原酸、绿原酸、儿茶素、表儿茶素、没食子酸乙酯、丹参素、阿魏酸、咖啡酸、没食子酸、原儿茶酸、原儿茶醛、迷迭香酸的含有量。方法该药物甲醇提取液的分析采用Waters Symmetry Shield RP C18色谱柱（3.9 mm×150 mm,5μm）;流动相乙腈-水（含0.1%甲酸）,梯度洗脱;体积流量0.4 m L/min;柱温20℃。结果 14种成分在各自范围内线性关系良好（r〉0.980 0）,平均加样回收率85.5%~107.0%,RSD 2.0%~5.0%。结论该方法简便、灵敏、准确、重复性好,可用于养血清脑颗粒的质量控制。

英文摘要：

AIM To establish an LC-MS/MS method for the simultaneous content determination of paeoniflorin,albiflorin,neochlorogenic acid,chlororogenic acid,catechin,epicatechin,ethyl gallate,danshensu,ferulic acid,caffeic acid,gallic acid,protocatechuic acid,protocatechualdehyde and rosmarinic acid in Yangxue Qingnao Granules（ Angelicae sinensis Radix,Chuanxiong Rhizoma,Paeoniae Radix Alba,etc.）. METHODS The analysis of methanol extract of this drug was performed on a 20 ℃ thermostatic Waters Symmetry Shield RP C18column（ 3. 9 mm × 150 mm,5 μm）,with the mobile phase comprising of acetonitrile-water（ containing 0. 1% formic acid） flowing at 0. 4 m L/min in a gradient elution manner. RESULTS Fourteen constituents showed good linear relationships within their own ranges,whose average recoveries were 85. 5%-107. 0% with the RSDs of 2. 0%-5. 0%. CONCLUSION This simple,sensitive,accurate and reproducible method can be used for the quality control of Yangxue Qingnao Granules.