中文摘要：

目的 观察尼古丁刺激大鼠气道平滑肌细胞（ASMC）后细胞内基础钙离子浓度的改变及平滑肌细胞膜瞬时受体电位通道（TRPC1,TPRC6）mRNA表达水平变化.方法 不同浓度尼古丁[O（对照组）、10、20、50、100μg/L]刺激大鼠气道平滑肌细胞,作用24及48 h后,采用Incyte细胞内钙浓度检测系统观察细胞内钙离子浓度的变化.提取细胞总RNA,反转录成cDNA,实时定量PCR方法检测细胞TRPC1和TRPC6 mRNA表达量变化.结果 20、50、100μg/L的尼古丁刺激大鼠气道平滑肌细24和48 h后,细胞内基础钙离子浓度均较对照组明显升高[（117.99±19.39）、（122.89±17.91）、（124.70±17.93）nmol/L比（85.85±12.60）nmol/L;（142.07±18.99）、（162.27±19.91）、（207.30±26.56）nmol/L比（98.04±2.39）nmol/L,均P＜0.05].而细胞中TRPC1和TPRC6 mRNA相对浓度也均较对照组显著升高（P＜0.05）,其中,100μg/L尼古丁刺激气道平滑肌细胞48 h后,细胞内基础钙离子浓度和TRPC6 mRNA相对表达量均较刺激24 h后的明显升高（P＜0.05）.结论 尼古丁可能通过上调大鼠气道平滑肌细胞膜瞬时受体电位通道TRPC1和TPRC6的表达而导致大鼠气道平滑肌细胞内基础钙离子浓度增加.

英文摘要：

Objective To study the changes of intracellular Ca2＋ concentration and transient receptor potential channels 1 and 6（TRPC1,TPRC6）mRNA expression in rat airway smooth muscle cells （ASMC） after nicotine intervention.Methods Various concentrations of nicotine（10 μg/L,20 μg,L,50μg/L,100μg/L）were used to stimulate rat airway smooth muscle cells.The intracellular Ca2＋ concentrations after nicotine intervention for 24 and 48 hours were measured by Incyte system.The total RNA was extracted from rat ASMC.and reversely transcribed into cDNA.The real-time PCR method was used to analyze the changes in TRPC1 and TRPC6 mRNA expression.Results The groups treated with different concentrations of nicotine（20 μg/L,50μg/L,100 μg/L）for 24 or 48 hours showed increases in intracellular Ca2＋concentration when compared with control[（117.99±19.39）,（122.89±17.91）,（124.70±17.93）nmol/L vs （85.85±12.60）nmol/L;（142.07±18.99）,（162.27±19.91）,（207.30±26.56）nmol/L vs（98.04±2.39）nmol/L;P〈0.05].In addition,relative expressions of TRPC1 and TPRC6 mRNA in rat ASMC also increased significantly compared with controls（P〈0.05）.The levels of intracellular Ca2＋ concentration and TRPC6mRNA were significantly higher with 48 h nicotine（100 μg/L,L）intervention than as found with 24 h nicotineintervention.Conclusion Nicotine may increase the intracellular Ca2＋ concentration by up-regulating the expression of TRPC1 and TPRC6 in rat ASMC.