中文摘要：

目的：研究羟基红花黄色素A（HSYA）联用β-乳香酸（β-BA）对血瘀证模型大鼠凝血功能、一氧化氮（NO）与环磷酸鸟苷（c GMP）的影响。方法：48只SD大鼠随机分为正常对照（等容生理盐水）组、模型（等容生理盐水）组、阿司匹林（100 mg/kg）组、HSYA（100 mg/kg）组、β-BA（200 mg/kg）组、联合用药（HSYA 50 mg/kg＋β-BA 100 mg/kg）组,每12 h灌胃给药1次,连续7次。第5次给药后给予大鼠皮下注射盐酸肾上腺素（0.8 mg/kg）2次,间隔4 h,中间给予冰水刺激以复制大鼠急性血瘀证模型。末次给药30 min内取腹主动脉血检测凝血指标和NO、c GMP水平,并取大鼠颈动脉观察其病理变化。结果：与正常对照组比较,模型组大鼠血浆凝血酶时间（TT）、血浆凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）缩短,纤维蛋白原（FIB）含量增加,NO、c GMP水平降低,差异均有统计学意义（P〈0.01）;病理切片表明,大鼠颈动脉内皮受损,血管内皮细胞部分从血管壁脱落。与模型组比较,HSYA组、β-BA组、联合用药组大鼠血浆TT、PT、APTT延长,FIB含量减少,NO、c GMP含量增加,差异均有统计学意义（P〈0.01）;大鼠血管内皮受损程度改善,且联合用药较单独用药效果明显。结论：HSYA联用β-BA能显著改变血瘀证大鼠的凝血功能,增加血浆NO与c GMP的含量。

英文摘要：

OBJECTIVE： To study the effect of HSYA （HSYA） and fl-boswellic acid （BA） on blood coagulation, nitric oxide （NO） and cyclic guanosine monophosphate （cGMP） in blood-stasis model rats. METHODS： 48 SD rats were randomly divided in- to normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, aspirin group （100 mg/kg） , HSYA group （100 mg/kg） , β-BA group （200 mg/kg） , drug combination group （HSYA 50 mg/kg＋β-BA 100 mg/kg）. They were given relevant medicine intragastrically every 12 h for consecutive 7 times. After the fifth administration, the model rats were given adrenalin hydrochloride（0.8 mg/kg） subcutaneously twice with an interval of 4 h. During the interval between two injec- tions, ice-cold water was used to induce acute blood stasis model. Blood samples were collected within 30 min after the last admin- istration to detect the coagulation parameters, NO and cGMP concentration; the pathological changes of carotid artery were ob- served. RESULTS： Compared with normal control group, the thrombin time （TT）, prothrombin time （PT） and activated partial thromboplastin time （APTT） were all shortened in model group; the content of fibrinogen content （FIB） increased while the con- centration of NO and cGMP decreased; there was statistical significance （P〈0.01）. Rats＇ vascular endothelium was impaired, and part of their vascular endothelial cell fell off from vascular wall. Compared with model group, TT and APTT were prolonged and the content of FIB decreased in HSYA group, β-BA group and drug combination group; there was statistical significance （P〈 0.01 ）.Vascular endothelium injury were improved, and the effect of drug combination was more obvious than single drug. CONCLU- SIONS： HSYA and β-boswellic acid can significantly improve coagulation function and increase NO and cGMP concentrations in blood-stasis model rats.