中文摘要：

目的研究二氮嗪预处理对原代培养海马神经元化学性缺氧再灌注损伤的保护作用。方法海马神经元分离培养10天后，随机分为4组：正常培养组，单纯缺氧组，预处理缺氧组，复合预处理缺氧组。后3组加入碘乙酸终浓度150mmol／L，孵育2．5h，再灌注，制备化学缺氧再灌注模型。预处理缺氧组缺氧前15min进行不同药物预处理。观察细胞形态，进行细胞死亡和凋亡的检测。结果正常培养组细胞死亡率（12．5±2．1）％，单纯缺氧组（80．5±3．8）％，差异有统计学意义（P〈0．01），顸处理缺氧组细胞死亡率明显降低（40．7±6．0）％，与单纯缺氧组比较，差异有统计学意义（P〈0．01），复合预处理缺氧组与单纯缺氧组比较无统计学差异（P〉0．05）。结论二氛嗪对海马神经元缺氧损伤具有明显的保护作用，5一羟基癸酸盐预处理可阻断二氮嗪的保护作用。

英文摘要：

Objective To investigate the protective effect of diazoxide pretreatment against chemical hypoxia/reoxygenation injury in primarily cultured hippocampal neurons. Methods Primarily cultured hippocampal neurons which were cultured for ten days were randomly divided into four groups： normal group, hypoxia group, diazoxide pretreatment group, diazoxide and 5-hydroxydecanoic acid（HD） pretreatment group. Neuronal cultures were subjected to metabolic poisoning by iodoacetic acid （IA）, followed by reperfusion to form chemical hypoxia-reperfusion model. In diazoxide pretreatment group and diazoxide and 5-HD pretreatment group, pretreatment with diazoxide or diazoxide and 5-HD was given 15 minutes before chemical hypoxia. Cell morphological change, neuronal viability and apoptosis were observed. Results Compared with normal group, the viability of hippocampal neurons in chemical hypoxia group was significantly decreased. Compared with chemical hypoxia group, the viability of neurons in diazoxide pretreatment group was increased,but the viability of neurons in the diazoxide and 5-HD pretreatment group was significantly decreased,and there was no obvious difference between hypoxia group and diazoxide with 5-HD pretreatment group. Conclusion Diazoxide pretreatment obviously protected hippocampal neurons against chemical hypoxia injury, and 5-HD blocked the protective effects of diazoxide pretreatment.