中文摘要：

实时荧光定量PCR（qRT-PCR）是目前基因定量表达分析的重要方法,而适宜内参基因的选择对目的基因表达特征的准确分析至关重要,研究目的是筛选适宜于红花檵木目的基因qRT-PCR分析的校正内参基因。以红花檵木‘大叶红’（Lorpetalum chinense var.rubrum‘Dayehong’）的芽、嫩茎、叶、花瓣、种子和愈伤组织为材料,采用qRT-PCR技术比较了4个常用看家基因微管蛋白基因（α-tubulin）、肌动蛋白基因（β-actin）、18S核糖体RNA（18SrRNA）与甘油醛-3-磷酸-脱氢酶基因（GAPDH）的表达情况。4个看家基因均能特异扩增并显示较高的扩增效率;经geNorm和NormFinder程序综合分析,当采用qRT-PCR分析红花檵木不同器官和组织中的基因表达差异时,以GAPDH表达最为稳定;在比较不同发育阶段的叶片及明暗处理的愈伤组织中的基因表达差异时,β-actin表达最为稳定。当采用qRT-PCR分析红花檵木不同器官和组织及不同发育阶段的叶片与明暗处理的愈伤组织基因表达差异时,可以分别选择GAPDH和β-actin作为校正内参基因。

英文摘要：

Real-time fluorescence quantitative PCR（qRT-PCR） has been widely used in gene expression analysis to date, and selection of suitable reference genes for qRT-PCR according to specific experimental materials or conditions is very important for accurate normalization of target gene expression. The goal of this study was to selection the suitable reference genes for qRT-PCR in L. chinense var. rubrum. In this study, expression of four commonly used housekeeping genes such as α-tubulin, β-actin, 18S ribsomal RNA （18S rRNA） and glyceraldehyde-3-phosphate dehydrogenase （GAPDI-1） were assessed by qRT-PCR in various tissues （buds, young stems, leaves, petals, seeds and callus）. All the candidate reference genes could amplify specifically with high efficiency in qRT-PCR reaction. To determine the expression stability of these genes, the data were determined by two commonly used applets （geNorm and NormFinder）, which produced highly comparable results depending on the experimental parameters. GeNorm and NormFinder algorithms revealed that β-actin and GAPDH were both in the highest stability. GAPDH could be used as a reference gene for qRT-PCR in various organs and tissues of L. chinense var. rubrum, and β-actin could be used as a reference gene for qRT-PCR in leaves at different stages and callus among diverse treatments of L. chinense var. rubrum.