文章采用稀释平板涂布法,研究了几种菌类抑制剂在菌根际土壤真菌及放线菌分离时的作用效果。试验选用6种抑制剂,每种抑制剂设4个浓度梯度,另外,设1个未加抑制剂的作对照。结果表明:在真菌分离培养过程中,阿米卡星浓度在0.05m l.L-1时分离出的真菌数量和种类分别达3.6×105个.g-1和13种,比未加抑制剂的分离效果还好。放线菌分离培养最佳抑制剂为重铬酸钾,重铬酸钾在浓度为10mg.L-1~30mg.L-1时对细菌的抑制作用均较好,并且对放线菌抑制作用很小,尤其在浓度为10mg.L-1时分离出的放线菌数量可达4.8×105个.g-1,与未加抑制剂的处理分离的数量无显著差异。
This paper investigated that a few inhibitors separating fungi and actinomycetes from mycorrhizal rhizosphere soil affected them in spreading dilutedness flat method.The examination selected six inhibitors,each inhibitors was four grads of concentration.In addition,the control did not add any inhibitors under the same condition.The result indicated when Amikacin s concentration was 0.05ml.L-1,the number and species of separating fungi were 3.6×105 entries.g-1 and 13 entries in separating and cultivating fun...