以青藏高原高寒草甸为研究对象,通过人工氮肥添加试验,研究6个群落优势种在不同施氮(N)水平下叶片碳(C)、N、磷(P)元素含量的变化以及生态化学计量学特征。结果表明:自然条件下,6个物种叶片N、P质量浓度存在显著的差异,表现为:黄花棘(Oxytropis ochrocephala)最高,为24.5和2.51g.kg-1,其叶片N含量低于而P含量高于我国其他草地的豆科植物:其余5个物种叶片N、P质量浓度分别为11.5-18.1和1.49—1.72g·kg-1,嵩草(Kobresia myosuroides)叶片N含量最低,垂穗披碱草(Elymus nutans)叶片P含量最低,与我国其他区域的研究结果相比,其叶片N和P含量均低于我国其他草地非豆科植物。随氮素添加量的增大,6种群落优势种叶片的C和P含量保持不变;其他5种植物叶片N含量显著增加,黄花棘豆叶片N含量保持不变。未添加氮肥时,6种植物叶片N:P为7.3-11.2,说明该区植物生长更多地受N限制。随N添加量的增加,除黄花棘豆外,其他5种植物叶片N:P大于16,表现为植物生长受P限制。综合研究表明,青藏草原高寒草甸植物叶片N含量较低,植物受N影响显著,但不同物种对N的添加反应不同,豆科植物黄花棘豆叶片对N添加不敏感,其他5个物种叶片全N含量随着N添加量的升高而增加.该研究结果可为高寨苴佃科学旆肥榀供王单诊依椐。
Aims Our purpose was to characterize the effects of nitrogen (N) addition on plant carbon (C), N, phosphorus (P), and C:N:P ecological stoichiometric characteristics in six dominant plant species, including Kobresia myosuroides, Elymus nutans, Anemone rivularis, Pedicularis kansuensis, Potentilla fragarioides and Oxytropis ochrocephala, of alpine meadow on the Qinghai-Xizang Plateau, China. Methods N was added at four levels. Concentrations of C, N and P were measured, and C:N:P was estimated in the six plant species following the N addition treatments. lmportantfindings Significant differences in leafN and P concentrations existed among the six species under natural conditions. The N and P concentrations were highest in (9. ochrocephala, at 24.5 and 2.51 g.kg-1, respectively. The leaf N concentration was significantly lower and leaf P concentration was significantly higher in O. ochrocephala than in legume plants of other grasslands in China. Leaf N and P concentrations in the other five species were in the ranges of 11.5-18.1 and 1.49-1.72 g.kg-1, respectively. Kokresia myosuroides had the lowest N concentrations and E. nutans had the lowest P concentrations; they were significantly lower than the non-legume plants in other grasslands in China (p 〈 0.001). P and C concentrations did not respond to N addition in all the six plant species, but N concentration significantly increased with N addition in five species other than O. ochrocephala, which did not respond to N addition. Values of the N:P varied in the range of 7.3-11.2 in treatment without N addition, indicating that the plant growth was limited by N in the alpine meadow. Values of the N:P increased and were greater than 16 with N addition in five species other than O. ochrocephala, indicating that N addition induced P deficiency in these five species. Our results point to very low leaf N concentration and limitation of N on plant growth in alpine meadow on the Qinghai-Xizang Plateau, China, but different species had differ