中文摘要：

建立同时测定花生油中4种黄曲霉毒素（包括黄曲霉毒素B1、B2、G1、G2）的液相色谱-串联质谱法（liquid chromatography-tandem mass spectrometry，LC．MS／MS）。该方法经甲醇一水、三氯甲烷依次提取样品，以C18柱分离，流动相为乙腈-0．1％甲酸溶液（梯度洗脱），采用电喷雾正离子多反应监测（multiple reactionmonitor，MRM）模式检测。结果表明：标准曲线在0．0～20．0μg／kg进样范围内线性良好；用本法测定4种黄曲霉毒素的回收率在70．8％-108．0％（低加标水甲）、82，4％4104．5％（中和高加标水甲）之间，相对标准偏差在5．7％～9．7％之间。运用所建立的方法对市售19种不同品牌和批次的花生油中的黄曲霉毒素进行分析，结果显示该方法选择性强、灵敏度高，适用于花生油中4种黄曲霉毒素的定性、定量测定。

英文摘要：

A liquid chromatography-tandem mass spectrometry （LC-MS/MS） method was established for simultaneous determination of 4 aflatoxins in peanut oil including aflatoxins B1, B2, G1 and G2. The sample preparation was achieved by sequential extraction with aqueous methanol solution and chloroform. The analytes were separated on a C18 column using a mobile phase composed of acetonitrile and 0.1% aqueous formic acid solution and detected using electrospray ionisation （ESI）- MS/MS in positive ion mode with multiple reaction monitoring （MRM）. The linear range of aflatoxins determination was from 0.0 to 20.0 μ g/kg. The recovery rates for four aflatoxins were from 70.8% to 108.0% at 1,0μg/kg and from 82.4% to 104.5% at 10.0 and 20.0 μ g/kg, and the relative standard deviations from 5.7% to 9.7%. The developed method proved highly selective and sensitive in determining 19 commercial peanut oil samples, thus being suitable for qualitative and quantitative analysis of four aflatoxins in peanut oil.