中文摘要：

目的探讨雌激素对大鼠肝癌肺转移形成的影响及其可能的机制。方法将雌性大鼠随机分为切除卵巢组、对照组、假手术组和去卵巢后加雌激素组,建立大鼠肝癌模型。诱癌20周后观察肿瘤发生、肺转移的情况;免疫组织化学法检测4组成瘤大鼠肝癌组织中雌激素受体α(ERα)、磷酸化雌激素受体α(P-ERα)、肝细胞生长因子(HGF)和白介素6(IL-6)的表达;半定量逆转录聚合酶链反应法检测ERα、HGF及IL-6的m RNA水平。结果切除卵巢组大鼠肝癌肺转移发生率明显高于其他3组(P<0.05);大鼠肝癌肺转移发生率越高,肝癌组织中HGF、IL-6的蛋白及分子水平表达越高(P<0.05),而ERα的蛋白及分子水平表达越低(P<0.05)。结论雌激素可能通过下调HGF和IL-6的表达来抑制肝癌肺转移的发生。

英文摘要：

Objective To determine whether and how estrogen reduced lung metastasis of HCC in a rat model. Methods We con-ducted a rat HCC model in female rats induced by diethylnitrosamine(DEN)and N-nitrosomorpholine(NMOR),and rats were distributed into four groups:ovariectomy,sham operation,ovariectomy followed by 30 μg/(kg·d)body weight/ day 17α-Ethynylestradiol(EE2)sup-plementation,sexually intact controls. Results Here we showed that,60% lung metastasis was observed in the rats of ovariectomy group, whereas obviously lower lung metastasis was found in the rats of other three groups. Furthermore,the higher expression of interleukin-6(IL-6), hepatocyte growth factor(HGF)and the lower expression of estrogen receptor α(ERα),the higher lung metastasis of HCC we founded. Con-clusion Estrogen may inhibit hepatic tumor lung metastasis by suppression of HGF and IL-6 production.