中文摘要：

PCR—DGGE技术是现代分子生物学技术中一种很重要的分析手段，近年来在环境微生物领域发展迅速，与传统微生物培养鉴定方法相比，PCR—DGGE技术简便、快速、准确，并且对于难以培养的微生物也具有较好的分离效果，因此，在环境微生物领域中受到普遍重视。介绍了PCRDGGE技术的基本原理、影响因素，以及在厌氧反应器中的应用，并对其应用前景做了展望。

英文摘要：

PCR-DGGE technique is a very important tool in modem molecular biology techniques in recent years.It has a rapid development in the field of environmental microbiology, compared to traditional identification of bacterial culture, PCR-DGGE technique is simple, rapid, accurate, and has a better separation effect to difficult culture organisms. Therefore, PCR-DGGE technique has received wide attention in the field of environmental microbiology. The basic principle and influence factors of this technology were introduced as well as application in the anaerobic reactor, and its application prospect was put forward.