中文摘要：

目的：原核表达 HBHAΔC 及 HBHAΔN 蛋白，比较 HBHA 系列蛋白活性。为进一步研究 HBHA 临床诊断价值提供实验依据。方法克隆 HBHAΔC 和 HBHAΔN 目的基因，用于大肠杆菌（E.coli）BL-21系统表达纯化。将蛋白通过 CL-6B 柱检测肝素结合能力。并加入到培养 BCG 的7H9液体培养基中，观察其诱导 BCG 聚集情况。结果 nHBHA，rHBHA 及HBHAΔN 蛋白均具有肝素结合能力。nHBHA、rHBHA 和 HBHAΔC 蛋白具备诱导 BCG 聚集的作用。结论成功获得 HBHA截短体蛋白；证实 HBHA 蛋白碳端参与肝素结合功能；蛋白氮端参与诱导 BCG 聚集反应。该实验结果为进一步研究 TB 感染分子机制及临床诊断应用奠定基础。

英文摘要：

Objective To express recombinant HBHAΔC and HBHAΔN protein,and compare the HBHA series protein activity with each other.It will be provide a experimental basis for the research on clinical diagnostic of HBHA.Methods The HBHAΔC and HBHAΔN gene fragments were cloned and expressed by transforming E.coli BL-21.Test the protein heparin binding ability by CL-6B column.And then added protein to the BCG 7H9 culture medium,to observe the induced BCG aggregation.Results nHB-HA,rHBHA and HBHAΔN protein have heparin binding ability.Meanwhile nHBHA,rHBHA and HBHA Δ C protein have in-duced BCG aggregation effect.Conclusion The HBHAΔC and HBHAΔN protein were successfully obtained.It was proved that the HBHA C-terminal could be combined with heparin and the N-terminal involved could induce the aggregation of BCG.This results provide a basis for further study on molecular mechanism of TB infection and clinical application.