中文摘要：

根据EST序列信息,利用RACE技术分离了巴西橡胶树（Hevea brasiliensis）的1个AP2/ERF类基因,命名为HbERFB4-1。该基因cDNA全长732 bp,含有完整的开放阅读框架,编码147个氨基酸,不含内含子。推导的HbERFB4-1氨基酸序列与杨树（Populus trichocarpa）、蓖麻（Ricinus communis）、拟南芥（Arabidopsis thaliana）和大豆（Glycine max）中相应蛋白氨基酸序列的一致性分别为78%、71%、62%和55%,具有典型的AP2类蛋白结构特征。聚类分析表明,HbERFB4-1属于ERF家族B4亚族。半定量RT-PCR分析结果表明,该基因在胶乳中的表达量相对较低,但乙烯利刺激后其在胶乳中的表达量迅速增加,推测该基因可能参与了胶乳中乙烯信号的转导。

英文摘要：

Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis,a full-length cDNA encoding AP2/ERF type gene named as HbERFB4-1,was cloned from H.brasiliensis by RACE-PCR.The HbERFB4-1 cDNA has full length of 732 bp with an open reading frame（ORF） of 444 bp,and encodes 147 amino acid residues.No introns were detected in HbERFB4-1gene.The deduced HbERFB4-1 contains an AP2 domain and three NISs,which shows high identity to AP2/ERF in Populus trichocarpa,Ricinus communis,Arabidopsis thaliana and Glycine max,counting for 78%、71%、62% and 55%,respectively.Phyogenetic analysis showed that HbERFB4-1 belonged to the B4 subfamily of ERF family.Semi-quantitative RT-PCR analysis indicated that the transcription of HbERFB4-1 in latex was low,but it was rapidly induced by ethephon,suggesting that HbERFB4-1 is involved in the ethylene signal pathway in latex of H.brasiliensis.