中文摘要：

根据EST序列信息，利用RACE技术从巴西橡胶树（Heveabrasiliensis）中克隆了1个NAC类转录因子基因HbNACl，其cDNA全长为1419bp，含有完整的开放阅读框，编码309个氨基酸。推导的氨基酸序列含有1个NAM结构域，具有典型的NAC类蛋白的结构特征，与毛果杨（Populus trichocarpa）、蓖麻（Ricinus communis）、水稻（Oryza sativa）和拟南芥（Arabidopsisthaliana）中的相应氨基酸序列的同源性分别达79％、80％、57％和60％。聚类分析表明，HbNAC1属于NAC家族Ⅱ亚族。半定量RT-PCR分析表明，该基因在胶乳中的表达较丰富，经乙烯利刺激后其在胶乳中的表达量明显增加。

英文摘要：

Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis, a full-length cDNA, named as HbNAC1, was cloned from H. brasiliensis by RACE-PCR, encoding a NAC transcription factor. The full-length of HbNAC1 cDNA was 1419 bp with an open reading frame （ORF） of 930 bp, and encoding 309 amino acids. The deduced HbNAC 1 with NAM domains had the typical characteristics of NAC family, and homologous to NACs in Populus trichocarpa, Ricinus communis, Oryza sativa and Arabidopsis thaliana of 79%, 80%, 57% and 60%, respectively. Phylogenetic analysis showed that HbNAC 1 was classified into theⅡ subfamily of NAC family. Semi-quantitative RT-PCR analysis indicated that the expression of HbNAC1 was rich in latex, and significantly enhanced by ethephon stimulation.