中文摘要：

Aurora-B是一种在细胞有丝分裂过程中起重要调控作用的丝氨酸/苏氨酸激酶,在哺乳动物卵母细胞减数分裂过程中的表达、定位与功能研究较少,相关猪的尚未见报道.本实验采用特异性抑制剂处理猪（Susscrofa）卵母细胞,研究其减数分裂过程中Aurora-B的表达、亚细胞定位与功能作用.激光共聚焦显微镜分析表明,Aurora-B均匀分布在生发泡（GV）期卵母细胞质中;生发泡破裂后,Aurora-B主要集中在浓缩染色质的周围,并随纺锤体的装配和染色体的分离而发生相应的迁移.使用Aurora-B特异性抑制剂AZD-1152处理后,阻滞在Pro M Ⅰ-AITI期卵母细胞与对照组相比有显著增加（P＜0.05）,MⅡ期卵母细胞显著降低（P＜0.05）,同时MⅡ期卵母细胞染色体的排列发生紊乱、纺锤体形态异常,并出现一定程度的微丝断裂和分散.抑制猪卵母细胞Aurora-B的表达,其孤雌胚胎的发育将受严重影响.本研究表明,Aurora-B可能在猪卵母细胞减数分裂过程中对于微管装配、染色体分离等,起着关键的调控作用.

英文摘要：

Aurora-B is a serine/threonine protein kinase which plays important regulatory role during mitotic cell cycle progression, but its expression, localization and functionality during mammalian oocyte meiosis areseldom reported, and no related reports in pigs （Sus scrofa）. In the present experiment, Aurora-B expression, subcellular localization, and its possible function during porcine oocyte meiotic maturation were investigatedvia confocal microscopic analysis and highly selective Aurora-B inhibitor treatment. Confocal microscopic analysis revealed that Aurora-B distributed uniformly in the cytoplasm at germinal vesicle（GV） stage. Aftergerminal vesicle breakdown, Aurora-B expression was mainly associated with chromosomal. During M Ⅰ period, with spindle assembly and sister chromatids were arranged in the equatorial plate, Aurora-B overlapedwith chromatin. In AITI, chromosomes began to migrate to the poles under the spindle traction, then Aurora-B located in the centre of chromosomes, but not overlaped with sister chromatids. Oocytes discharged the firstpolar body（pbI） after 44 hours when Aurora-B enriched on the oocyte nucleus and pbI. After using inhibitors AZD-1152 treatment, the results showed that more and more oocytes arrested in GV stage with the increasingconcentrations of AZD-1152, GVBD incidence suppressed; compared with control group, the oocytes arrested in Pro M Ⅰ -AITI were significantly increased （P〈0.05）, arrested in M Ⅱ were significantly reduced（P〈0.05）. Atthe same time, M Ⅱ oocyte chromosome misaligned, some failed to align; abnormal spindle morphology, even disappeared; actin filaments had a certain degree of fracture and dispersion. In addition, porcine parthenogeneticembryos development severely affected after suppressing the expression of Aurora-B by inhibitor on oocytes.Cleavage rate, blastocyst rate and the total number of blastocyst cells are significantly lower with the increasing concentrations of AZD-1152 in each treatment group, and no blastocyst when the