中文摘要：

【目的】通过优化产琥珀酸放线杆菌GXAS137同步糖化发酵木薯粉产丁二酸的发酵培养基,提高丁二酸产量,降低生产成本。【方法】在单因素试验的基础上,先利用Plackett-Burman试验设计筛选出影响丁二酸发酵的重要参数,再采用正交试验确定重要参数的最佳水平。【结果】价格低廉玉米浆可用作氮源,影响丁二酸产量的重要参数是木薯粉、玉米浆、碱式碳酸镁和糖化酶浓度。最佳条件为（g/L）：木薯粉100,玉米浆14,糖化酶2.0 AGU/g底物,碱式碳酸镁75。优化后丁二酸产量达到69.31 g/L,丁二酸得率为90.01%,生产强度为1.44 g/（L·h）。与初始条件（52.34 g/L）相比,丁二酸浓度提高了32.42%。并利用1.3 L发酵罐对SSF与SHF两种发酵工艺进行了比较,SSF丁二酸产量（72.21 g/L）远高于SHF（56.86 g/L）。【结论】产琥珀酸放线杆菌同步糖化发酵木薯粉丁二酸产量高,生产成本低,具有较好的工业化应用前景。

英文摘要：

[Objective] In order to succinic acid production from raising yields Actinobacillus and reducing production costs, succinogenes GXAS 137 by we optimized simultaneous saccharification and fermentation （SSF） with cassava flour as carbon source. [Methods] The important parameters were screened by the single factor experiment and Plackeet-Burman design. Then, the optimum values of the parameters were obtained by orthogonal experiment design. [Results] The results showed that corn steep liquor （CSL） was used as a proper nutrient in the succinic acid production from cassava flour. The optimum medium compositions were determined as （g/L）： cassava flour 100, CSL 14, glucoamylase dose 2.0 AGU/g substrate and MgCO3 75. The succinic acid yield reached 69.31 g/L at the optimal condition. The conversion rate of cassava to succinic acid reached 90.01% and succinic acid productivity was 1.44 g/（L.h）. The succinic acid content increased by 32.42% than that without the optimized condition （56.86 g/L）. The simultaneous saccharification fermentation （SSF） and separated hydrolysis and fermentation （SHF） were compared with 1.3 L fermenter. The results showed that the succinic acid of SSF （72.21 g/L） was superior than SHF. [Conclusion] The high-level succinic acid production and low production cost from cassava flour may facilitate industrial scale application in future.