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血管紧张素(1—7)对血管紧张素Ⅱ诱导肝星状细胞表达结缔组织生长因子的抑制作用
  • ISSN号:1007-3418
  • 期刊名称:《中华肝脏病杂志》
  • 分类:R692[医药卫生—泌尿科学;医药卫生—临床医学;医药卫生—外科学]
  • 作者机构:[1]南方医科大学南方医院急诊科,广州510515, [2]南方医科大学南方医院呼吸科,广州510515, [3]福建省武警总队医院
  • 相关基金:基金项目:国家自然科学基金(30770974,30871155);
中文摘要:

目的探讨血管紧张素(Ang)(1-7)对AngⅡ诱导的肝星状细胞(HSC)表达结缔组织生长因子(CTGF)的抑制作用及其机制。方法培养人HSC细胞系(LX2细胞),以AngⅡ刺激24h,分别予以AngⅡ1型受体阻断剂irbesartan、ROCK抑制剂Y27632预处理1h。设立AngⅡ+Ang(1—7)处理组,观察Ang(1—7)对AngⅡ的抑制作用;Mas受体拮抗剂A779阻断Mas受体,观察对Ang(1—7)抑制效应的阻断作用。利用Western blot、逆转录聚合酶链反应(RT-PCR)和多基因定量检测系统检测RhoA、ROCK2 mRNA,结缔组织生长因子(CTGF)蛋白和mRNA的表达。结果研究结果显示AngⅡ刺激后,RhoA蛋白(0.87±0.093对比0.337±0.074)、ROCK2mRNA(0.747±0.061对比0.163±0.024)、CTGFmRNA(0.578±0.028对比0.262±0.007)相对表达量与对照组相比显著增高,差异有统计学意义(P值均〈0.01);irbesartan(RhoA蛋白:0.558±0.030对比0.87±0.093;ROCK2mRNA:0.368±0.023对比0.747±0.061;CTGFmRNA:0.309±0.019对比0.578±0.028)、Y27632(RhoA蛋白:0.564±0.067对比0.87±0.093;ROCK2mRNA:0.218±0.046对比0.747±0.061;CTGFmRNA:0.340±0.020对比0.578±0.028)预处理组上述基因或蛋白相对表达量显著减低,差异有统计学意义(P值均〈0.01),Ang(1-7)单独处理组RhoA蛋白(0.449±0.035对比0.337±0.074)及CTGFmRNA(O.256±0.008对比0.262±0.007)与对照组相比无明显改变,差异无统计学意义(P值均〉0.05);但是Ang(1-7)可抑制AngⅡ诱导的上述基因或蛋白表达的增高(RhoA蛋白:0.615±0.034对比0.87±0.093IROCK2mRNA:0.403±0.040对比0.747±0.061;CTGFmRNA:0.265±0.011对比0.578±0.028),差异有统计学意义护值均〈0.01);Mas受体拮抗剂A779可以阻断Ang(1-7)对AngⅡ的抑制作用(RhoA蛋白:0.929±0.076对比0.615±0.

英文摘要:

Objective To explore the angiotensin peptide [Ang (1-7)]-mediated inhibition of AngⅡ in human hepatic stellate cells (HSCs) and determine the involvement of the ACE2-Ang (1-7)-Mas axis.Methods The human HSC line, LX2, was used in all experiments, and divided into control (unstimulated) and AngⅡ-stimulated (10-6 mol/L) groups. The Ang H-stimulated cells were further divided among several pre- treatment (prior to Ang ID groups: ROCK-inhibited (Y27632 blocking agent, 10-6 mol/L); irbesartan-inhibited (AT-1 receptor antagonist, 10-6 mol/L); and Mas receptor-inhibited (A779 Mas receptor antagonist, 10-6 mol/L). To explore the potential inhibitory effects of various Ang family members, the AngⅡ-stimulated and pre-treated LX2 cells were exposed to Ang (1-7) (10-6 mol/L) for 24 h. Western blot, reverse transcription-polymerase chain reaction (RT-PCR), and QuantiGene assay were used to assess changes in protein and mRNA expression levels of RhoA, ROCK, and connective tissue growth factor (CTGF). Results Compared with the control group, AngⅡ- stimulated cells showed significantly increased levels of RhoA protein (0.337 ± 0.074 vs. 0.870 ± 0.093), ROCK2 mRNA (0.747 ± 0.061 vs. 0.368 ± 0.023), and CTGF mRNA (0.262 ±0.007 vs. 0.578 ± 0.028) (all, P〈0.01). Pre-lreatment with irbesartan or Y27632 eliminated these responses. Ang (1-7) inhibited the Ang H-stimulated up- regulation of RhoA, ROCK, and CTGF. Conclusion Ang (1-7) can inhibit the AngⅡ-stimulated up-regulation of RhoA, ROCK and CTGF in hepatic stellate cells, indicating that the ACE2-Ang (1-7)-Mas axis, an important branch of the renin-Ang-aldosterone system is involved in the occurrence and development of liver fibrosis.

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期刊信息
  • 《中华肝脏病杂志》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中华医学会
  • 主编:
  • 地址:重庆市渝中区临江路74号
  • 邮编:400010
  • 邮箱:chnhepa@online.cq.cn
  • 电话:023-63706512
  • 国际标准刊号:ISSN:1007-3418
  • 国内统一刊号:ISSN:50-1113/R
  • 邮发代号:78-56
  • 获奖情况:
  • 中国期刊方阵“双效”期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),荷兰文摘与引文数据库,美国生物医学检索系统,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:47128