中文摘要：

将难溶性的锌卟啉（ZnTpHPP）与牛血清白蛋白（BSA）结合,制得一类新型水溶性生物高分子金属卟啉配合物（BSA-ZnTpHPP）.通过紫外可见光谱（UV-Vis）、圆二色谱（CD）及非变性聚丙烯酰胺凝胶电泳（Native-PAGE）对BSA-ZnTpHPP的结构进行了表征,发现二者以配位键结合,BSA与锌卟啉以较低比例结合时蛋白质二级结构保持.考察了BSA-ZnTpHPP的光敏感性,发现BSA-ZnTpHPP在光照条件下易变成三重激发态,可以将电子转移给甲基紫精（MV2＋）.以三乙醇胺（TEOA）为电子供体,甲基紫精（MV2＋）为电子中继体,以BSA-ZnTpHPP/MV2＋/TEOA/胶体Pt四组分体系考察了BSA-ZnTpHPP的光诱导水解产氢性能,结果表明,这类水溶性生物高分子金属卟啉光敏剂具有良好的光解水产氢性能.

英文摘要：

A novel water soluble biopolymer metalloporphyrin complex（BSA-ZnTpHPP） was prepared by combination of insoluble meso-tetra（4-hydroxyphenyl） porphyrin zinc complex（ZnTpHPP） and bovine serum albumin（BSA）.The complex was characterized by UV-Vis spectroscopy,circular dichroism,and native polyacrylamide gel electrophoresis.The secondary structure of BSA was maintained after coordination of the metalloporphyrin complex at a low ratio.The photosensitivity of BSA-ZnTpHPP was also investigated.Its fluorescence was quenched by methyl viologen（MV2＋）,which suggests that photo-induced electron transfer occurs from BSA-ZnTpHPP to MV2＋ in the BSA-ZnTpHPP/MV2＋/triethanolamine（TEOA） system.Under light,BSA-ZnTpHPP was easily excited to the triplet state,and then transferred an electron to MV2＋.The BSA-ZnTpHPP/MV2＋/TEOA/colloidal Pt system was applied to photosensitized reduction of water for preparation of hydrogen.The water-soluble biopolymer metalloporphyrin complex was an excellent biopolymer photosensitizer.