中文摘要：

将经福尔马林灭活的嗜水气单胞菌（Aeromonas hydrophila）、菌体脂多糖（LPS）和菌体外膜蛋白（OMP）作为免疫原,分别接种斑点叉尾（Ictalurus punctatus Rafinsque）后,通过测定受免鱼的凝集抗体效价,头肾和血液中吞噬细胞的吞噬活性和用A.hydrophila活菌攻毒的方法,探讨了斑点叉尾对A.hydrophila的3种疫苗的免疫应答状况和对活菌攻毒的免疫保护效果。试验结果显示：对斑点叉尾经腹腔注射接种3种疫苗均能刺激受免鱼产生较强的免疫应答,3种疫苗的受免鱼均产生了特异性凝集抗体,接种F-Ah灭活菌苗的试验鱼最高,接种OMP疫苗的试验鱼其次,而接种LPS疫苗的试验鱼血清中凝集抗体效价最低;与未接种疫苗的对照鱼相比,受免鱼头肾和血液中吞噬细胞的吞噬活性明显上升,头肾和血液中吞噬细胞活性由高到低依次是LPS、OMP和F-Ah免疫接种的斑点叉尾;活菌攻毒的结果证明接种3种疫苗的受免斑点叉尾A.hydrophila的感染产生了不同程度的相对免疫保护力（RPS）,以OMP免疫接种后的斑点叉尾RPS最高,达到72.5%,接种LPS的斑点叉尾稍差,RPS为62.5%,而RPS最低的是接种F—Ah的免疫组，RPS只有24．2％。

英文摘要：

Channel catfish（Ictalurus punctatus Rafinsque） were immunized with three vaccines（formalin killed Aeromonas hydrophila：F-Ah,lipopolysaccharide：LPS and outer membrane protein： OMP）.The immune response against three vaccines and protective immunity by challenging with live A.hydrophila of channel catfish were studied by determining agglutination antibody titers and phagocytic activity of phagocyies in head kidney and blood,and challenging with live A.hydrophila.The result showed that all 3 vaccines could stimulate stronger immune response in the immunized channel fish and all channel fish immunized by 3 vaccines produced specific agglutination antibody,while fish immunized by F-Ah was the highest,followed by OMP,LPS was the least.Phagocytic activity of phagocyies in head kidney and blood of immunized fish increased significantly than control group,the highest was LPS,followed by OMP,F-Ah was the lowest.The result of challenging with live A.hydrophila showed that all channel catfish immunized by 3 vaccines had relative percent survival（RPS）at different degree.OMP had the highest RPS,which was 72.5%,followed by LPS（62.5%） and F-Ah（24.2%）.