中文摘要：

以荧光物质铽的配合物为核，二氧化硅为外壳，制备了大小均匀的新型稀土配合物荧光纳米粒子。实验采用油包水的反相微乳液法，在四乙氧基硅烷和3-氨丙基三乙氧基硅烷（APTEOS）的共水解下进行。通过透射电子显微镜（TEM）观察，所得的纳米粒子呈球形，大小均匀，直径在30nm左右。纳米粒子呈现很强的铽配合物发光，且发光性质十分稳定。可通过表面的氨基方便地与生物分子偶联，可作为一种优秀的时间分辨荧光标记物。

英文摘要：

Novel silica-coated terbium （Ⅲ） chelate fluorescent nanoparticles were prepared and characterized as a new type of fluorescence probe for highly sensitive bioassay. The preparation was carried out in a water-in-oil （W/O） microemulsion containing a chelate（pAB-DTPAA-APTEOS）, Triton X-100, hexanol, and cyclohexane by controlling copolymerization of tetraethyl orthosilicate （TEOS） and 3-aminopropyl-triethyloxysilane （APTEOS）. The nanoparticles are spherical and uniform in size, about 30 nm in diameter, strongly fluorescent, and highly stable. The amino groups directly introduced to the nanoparticles surface by using APTEOS in the preparation made the surface modification and bioconjugation of the nanoparticles easier. The method significantly reduces fluorescent compounds leaching from the nanoparticles. The particles are potential of good biocompatibility and can be excepted as efficient biological labels.