中文摘要：

通过合成丹酰氯的荧光单体硅酯前驱物，采用油包水的反相微乳液法，以丹酰氯的荧光单体硅酯前驱物为核材料，成功地制备了丹酰氯的荧光纳米颗粒，克服了传统方法制备核壳荧光纳米颗粒中存在的荧光染料泄漏问题。通过透射电子显微镜表征该纳米粒子呈球形，大小均匀，直径为40nm左右。所制得纳米颗粒荧光性质稳定，受外界环境的影响小，且潜在生物亲和性，是一种新型的荧光标记物。

英文摘要：

In an effort to prepare efficient and uniform biolabels, we have developed a novel fluorophore doped silica nanoparticles via water-in-oil （W/O） microemulsion method. The controlled hydrolysis of tetraethyl orthosilicate （TEOS） in W/O microemulsion leads to the formation of monodisperse fluorophore doped silica nanoparticles. By synthesizing of monomer dansyl-Cl （DNS-Cl） silica precursor, we have successfully prepared fluorescent hybrid silica nanoparticles of DNS-Cl. The fluorophores are well protected from the environment when they were doped inside the silica network. The transmission electron microscope （TEM）photograph showed that the particles are very uniform, they are spherical and monodispersed with a diameter of about 40 nm. The excitation maximum and emission maximum, compared with the monomer precursor have a blue-shift. The fluorescence intensity of the fluorophore doped silica nanoparticles passed through a maximum with increasing monomer precursor concentration,the optimal loading is 100 μL of monomer precursor. The effect of pH on monomer precursor is very distinct, both the fluorescence intensity and the excitation wavelength have changed a lot, but the fluorophore doped silica nanoparticles are nearly insusceptible, which indicates that core-shell structure reduces the effect of the environment on fluorophores. The method significantly reduces fluorescent dye leaching from the nanoparticles too, since there is a firm chemical bond between DNS-CI and APTEOS, the particles are potential of good biocompafibility, since they have a pure silica surface and can thus be modified easily with many biomolecules for added biochemical functionality. The particles can be accepted as an effective biological labels.