中文摘要：

目的观察在体外涂刷细胞黏附剂（cell adherent reagent,CAR）对羟基磷灰石（biphasic calcium phosphate,BCP）生物学活性的影响。方法成骨细胞被种植入经过CAR涂刷的BCP材料（实验组,即BCP-CAR组）和用生理盐水处理过的BCP材料（空白组,即BCP组）中,在培养2d、5d、8d后,分别测定细胞增殖、细胞生存活力、碱性磷酸酶（ALP）活性,RT-PCR用于检测成骨细胞runt转录相关因子2（RUNX2）、ALP、骨唾液蛋白（BSP）和Ⅰ型胶原酶（COLⅠ）的基因表达。结果与空白组对比,涂刷了CAR的BCP材料,其成骨细胞的细胞增殖和ALP活力上调,RUNX2、BSP、ALP及COLⅠ基因表达上调,差异均有统计学意义（P〈0.05）。结论经过CAR涂刷能增强BCP材料的生物活性,这为其临床应用提供了重要的依据。

英文摘要：

Objective This study aim is to observe the effects of the biphasic calcium phosphate （BCP） ce- ramics coated with cell adherent reagent （CAR） on biological activity of BCP ceramics in vitro. Methods Os- teoblasts were cultivated on BCP ceramics administered with normal saline （the blank group or BCP group） and on BCP ceramics coated with cell adherent reagent （the experimental group or BCP/CAR group）. On day 2, day 5 and day 8 of cultivation, the detection of cell proliferation, cell viability, alkaline phosphatase （ALP） ac- tivity were performed, respectively; and expressions of osteoblastic ALP, bone sialoprotein （BSP）, type I col- lagen （COLI） genes and runt--related transcription factor 2 （RUNX2） were measured by using Reverse Transcriptase Polymerase Chain Reaction. Results In the experimental group： the osteoblastic cell proliferation and ALP activity were upregulated;and expressions of RUNX2, BSP, ALP and COL I genes were upreg- ulated. Comparison with the blank group showed there were all statistically significant differences （ P 〈0.05）. Conclusion BCP coated with CAR can promote the biological activity of BCP and this conclusion provides important evidence for its clinical use.