中文摘要：

目的利用细胞及分子生物学技术，研究白纹伊蚊贵州省贵阳、兴义、毕节株对1—4型登革病毒（DEN1—4）的垂直传递能力。方法采集贵州省贵阳、兴义、毕节市市郊自然界白纹伊蚊，驯化3代。分别取羽化后3～5d龄成蚊为实验组，白纹伊蚊海南株为对照组；用DEN1—4国际参考株分别感染各组蚊虫，将感染后亲代及子1～3代成蚊制备蚊悬液，接种C6／36进行病毒分离并鉴定；间接免疫荧光法检测DEN抗原；常规方法提取总RNA。利用DENNS1基因区通用引物经逆转录-聚合酶链反应（RT—PCR）检测DEN核酸，限制性内切酶酶切分型。结果白纹伊蚊贵阳、兴义、毕节、海南株亲代蚊虫均对DEN易感；白纹伊蚊兴义株与海南株均能垂直传递DEN-1、4到子3代。传递DEN-3到子2代，白纹伊蚊兴义株能垂直传递DEN-2到子3代，白纹伊蚊贵阳．株能垂直传递DEN-1到子3代，传递DEN-2、3、4到子2代，白纹伊蚊毕节株能垂直传递DEN-1到子2代，DEN-4至子3代，未发现能垂直传递DEN-2、3。结论对DEN1—4易感的白纹伊蚊贵阳、兴义、毕节株能垂直传递DEN，且对DEN的垂直传递能力与其地理来源有关；贵州省3个地方株的白纹伊蚊卵可在干燥室温条件下越冬保存DEN。

英文摘要：

Objective To study vertical transmission capability of dengue virus type 1 - 4（DEN 1 - 4） through three geographic strains Aedes albopictus in Guizhou province. Methods Three geographic strains of Aedes albopictus in Guizhou Province were infected orally with DEN1 - 4, respectively. Parental and F1 - F3 generation mosquitoes were gathered. Dengue virus was isolated with C6/36 ; The viral antigen was detected by indirect immunofluore sences assay （IFA）. The total RNA was obtained , NS1 gene of DEN was detected by RT - PCR. Results Three Guizhou geographic mosquitoes of Aedes albopictus eould be infected orally with DEN1 - 4; Both Xingyl and Hainan geographic mosquitoes had the capbility of vertical transmission DEN- 1 and DEN - 4 to F3 generation and DEN - 3 to F2 generation as well, Xingyi geographic mosquitoes were detected vertical transmission DEN- 2 to F3 generation; Guiyang geographic mosquitoes eould vertically transmit DEN - 1 to F3 generation, DEN- 2, DEN - 3 and DEN - 4 to F2 generation;Transmitted DEN - 1 to F2 generation, DEN - 4 to F3 generation, transmitted DEN - 2 and DEN - 3 to progeny were not be detected by Bijie mosquitoes. Conclusion Three geographic strains Aedes albopictus in Guizhou were susceptible to dengue virus type 1 -4; Guiyang, Bijie and Xingyi geographic mosquitoes of Aedes Aalbopictus eould vertical transmit DEN, and their efficiency were various. The eggs of filial mosquitoes eould llve through the winter at dry, room temperature.