本实验以 ICR小鼠为实验材料,通过脑组织总 mRNA 提取、RT-PCR 的方法分别扩增目的片段 rcan2-L 和rcan2-S,并将其构建到 pEGFP-C1质粒中,获得序列正确的、用于真核细胞表达的重组质粒 pEGFP-C1/rcan2-L 和pEGFP-C1/rcan2-S,为进一步深入研究 RCAN2的功能奠定了基础。
This experiment aimed at constructing eukaryotic expression plasmids.ICR mice were used, brain tissue total RNA was extracted,target fragment rcan2-L and rcan2-S were amplified,built into pEGFP-C1 plasmid. The obtained recombinant plasmids pEGFP-C1/rcan2-L and pEGFP-C1/rcan2-S will help in elucidating the function of RCAN2 s.