中文摘要：

从小鼠脑组织中通过总mRNA的提取、RT-PCR方法，利用pEASY-T1载体克隆出了鼠源rcan1-11基因，并构建出了重组有rcan1-11基因的PET21a原核表达质粒，进行了表达条件的初步摸索，这为今后研究rcan1-11基因以及蛋白的结构与功能打下了前期实验基础．

英文摘要：

This experiment was aimed at constructing prokaryotic and eukaryotic expression plasmids. Rcanl-1 genes were cloned from mouse brain by RT-PCR. Recombinant clones were selected by blue white plaque assay and PCR screen after target gene was subcloned into pEASY-T1 cloning vector. Sequencing confirmed that rcanl-1 clone from mouse brain was correct; therefore prokaryotic PET21a plasmid was constructed using restriction enzymes. The right recombinant plasmid was induced to express in expression culture, with the expressed protein checked by SDS-PAGE and Western blot. This work will to facilitate additional studies on RCANI-IL protein structure and function.