中文摘要：

针对已筛选获得的甲烷氧化混合菌,选取填埋场覆盖土（LCS）、矿化垃圾（AR）和塘渣（TZ）3种填埋场周边易得的材料为供试生存基质,从基质选择性及长效性角度进行了甲烷减排应用条件的探究及使用效能评估.结果表明,在TZ、LCS、AR、TZ-AR和LCS-AR这5种生存基质中,TZ-AR最适合甲烷氧化混合菌的生长,且TZ与AR的复配比例以5∶5为最佳.甲烷氧化混合菌在TZ-AR的粒径≤4 mm和含水率为20%时具有最高甲烷氧化能力.一次性接种甲烷氧化混合菌在静态体系中的最佳使用有效期为31 d.其在接种量为0.08、0.16、0.20 mL·g^-1和0.25m L·g^-1时甲烷氧化速率无明显差异,从工程应用角度而言,8%的接种量为最佳.

英文摘要：

Three kinds of available typical materials, landfill cover soil （ LCS）, aged refuse （AR） and pong residue （TZ） , were chosen as substrates to study the CH4 emission application condition in terms of the selectivity and long-term performance of cultivate substrate for isolated methanotrops （ mixed bacteria）. It is shown that the combination of TZ-AR with the TZ-to-AR ratio of 5：5 was optimal for the growth of metbanotrophs （mixed bacteria） among the tested substrates. The highest methane oxidation capacity was observed when the TZ-AR particle size is no more than 4 mm and the moisture content is 20%. The validity of methanotrophs （ mixed bacteria） can last for 31 days when inoculated once under statistic state. From the viewpoint of engineering application, the optimal inoculation ratio for methanotrophs （mixed bacteria） is 0.08 mL. g^-1 as there are no significant difference on methane oxidation rate for 0.08, 0.16, 0.20 mL.g^-1 and 0. 25 mL.g^-1.