中文摘要：

选取了湖泊底泥、矿化垃圾、覆盖土和好氧污泥4种填埋场周边易得基质为分离源,采用驯化、传代培养、甲烷氧化能力及适应性等方法进行了适合于填埋场覆盖层应用的甲烷氧化菌的筛选研究.结果表明,从好氧污泥中分离得到的甲烷氧化菌较其它分离源得到的甲烷氧化菌具有更高的甲烷氧化活性（2461.29μg·h-1·g-1,以每g干污泥每小时氧化的CH4量（μg）计,下同）和甲烷氧化速率增幅（1045.56μg·h-1·g-1,以每g干污泥每小时氧化的CH4量（μg）计,下同）,接种后能显著提高覆盖材料的甲烷氧化能力（p〈0.05）,且不受CH4浓度的抑制.源自好氧污泥的甲烷氧化菌具有生长迟滞期短、生长速率高等特点,对填埋场的甲烷削减更具适应性,有利于工程扩大应用.

英文摘要：

Four available typical substrates including lake sediment, aged refuse, landfill cover soil and aerobic sludge were chosen as initial inoculums to isolate methanotrophs （mixed bacteria） by using domestication, subculture, methane oxidation capacity and adaptability tests. It shows that methanotrophs （mixed bacteria） isolated from the aerobic sludge from wastewater treatment plant has the highest methane oxidation capacity of 2461.29 Ixg （CH4 ） ~ h-L g-1 （dw） and incremental rate of 1045.56 Ixg （CH4） ＂h-1＂g-1（dw） among the four tested substrates. It can significantly improve the cover layer＇s methane oxidation capacity which is not limited by the methane concentration after inoculation. Methanotrophs （mixed bacteria） isolated from aerobic sludge is more suitable for engineering application for methane reduction in landfill due to their short growth lag phase and fast growth rate.