采用9个重复性好的随机引物对被子植物上的28个散斑壳属(Lophoderm iumChevall.)菌株进行RAPD-PCR扩增,通过RAPD分析结果结合表型性状的比较和分析,确定了子囊果埋生位置,子座、子囊、子囊孢子、侧丝等形态学特征以及寄主种类差异在该类菌物种水平分类上的重要性,另外产地不同等对种内遗传多样性产生一定影响。进一步明确了被子植物生散斑壳属一些种内和种间的亲缘关系。
Twenty-eight Lophodermium strains on angiosperms were charaterized by RAPD reaction with 9 random primers whose gel electrophoresis diagrams can be amplified repeatedly and clearly.By the comparison of RAPD analytic results and the phenotypic characters,the importance of some morphological characters such as the embedded position of ascomata,stromata,asci,ascospores,paraphyses and the category of hosts on the classification of species level were determined.Relationships of some intraspecific and interspecific strains of Lophodermuim on angiosperms were affirmed.