以交联的魔芋葡甘聚糖微球(KGM)为基质,制备了DEAE型的阴离子交换层析介质,考察了反应物浓度、温度、时间等反应因素对魔芋葡甘聚糖层析介质离子交换容量的影响,确定最优反应参数。结果表明,层析介质的离子交换容量达到3.42mmol/g,蛋白吸附容量高达126.84mg/mL,蛋白洗脱率为90.42%。
DEAE anion exchangers were prepared by crossing diethylaminoethyl (DEAE) onto konjac glucommannan gel. Effects of reactants concentration, reaction temperature, reaction time and other reaction factors on exchange capacity of konjac glucommannan gel were studied and optimized reaction parameters were obtained. Under the optimum reaction condition, the exchange capacity of the prepared gel was 3.42mmol/g, the adsorption capacities for protein was 126.84mg/mL. and the desorotion reached 90.42%.