中文摘要：

目的观察过氧化氢（H2O2）及转化生长因子（TGF）-β2诱导人小梁网细胞（HTMCs）后对纤维连接蛋白（FN）、胶原蛋白1型（COL1）、核因子（NF）-κBP65蛋白和白细胞介素（IL）-1β基因表达的影响及白藜芦醇（RSV）的干预作用。方法（1）选取汇合度70%～80%的HTMCs分为5组。实验组于无血清培养基中分别加入浓度为150、300、450、800μmol/L的H2O2处理，对照组的培养基中不加H2O2。Westernblot法检测各组FN、COL1、NF-κBP65、NF-κBP65磷酸化（P-NF-κBP65）蛋白的表达，实时定量PCR法检测IL-1β基因的表达。（2）HTMCs细胞分为3组。对照组以不含H2O2及RSV的无血清培基处理，H2O2组以300μmol/L的H2O2处理，H2O2+RSV组同时加入300μmol/L的H2O2及25μmol/L的RSV处理。检测各组上述蛋白和基因的表达情况。免疫荧光检测各组NF-κBP65在HTMCs中的定位。（3）HTMCs细胞分为3组。对照组以不含TGF-β2及RSV的无血清培基处理，TGF-β2组以5μg/L的TGF-β2处理，TGF-β2+RSV组为同时加入5μg/L的TGF-β2及25μmol/L的RSV处理。检测各组上述蛋白和基因的表达情况。结果（1）与对照组比较，150、300、450、800μmol/L组FN和P-NF-κBP65蛋白表达水平均增高，300、450、800μmol/L组COL1蛋白和IL-1β基因表达水平增高（P<0.05），其他指标比较差异均无统计学意义。（2）H2O2组较对照组FN、COL1、P-NF-κBP65蛋白和IL-1β基因表达水平均增高，而H2O2+RSV组较H2O2组上述指标均降低，H2O2+RSV组较对照组仅IL-1β降低（P<0.05）。对照组仅细胞质表达NF-κBP65，H2O2组细胞胞质及核中均有NF-κBP65表达，且核中表达较多；H2O2+RSV组细胞胞质中表达NF-κBP65较核中多。（3）TGF-β2组较对照组FN、COL1、P-NF-κBP65的蛋白和IL-1β基因水平表达均增高（P<0.05），TGF-β2+RSV组较TGF-β2组上述指标均降低（P<0.05）。结论H2O2和TGF-β2能上调HTMCs的FN、COL1、P-NF-κBP65蛋白及IL-1β基因的?

英文摘要：

Objective To investigate hydrogen peroxide (H2O2) and transforming growth factor-β2 (TGF-β2) induced fibronectin (FN), collagen 1 (COL1), nuclear factor (NF) -κB P65 proteins and interlukin (IL) -1β gene expression in human trabecular meshwork cells (HTMCs), and the interventional mechanism of resveratrol (RSV). Methods (1) HTMCs with 70 to 80% confluency were divided into 5 groups. The experimental groups were treated with serum-free medium and with H2O2 at concentrations of 150, 300, 450 and 800 μmol/L. The control group was treated with 0 μmol/L H2O2. The protein levels of FN, COL1, NF-κB P65 and NF-κB P65 phosphorylation (P-NF-κB P65) were measured by Western blot assay. The expression of IL-1β gene was measured by qPCR. (2) HTMCs were divided into 3 groups. The control group was treated with serum-free medium and without H2O2 and RSV. The H2O2 group was treated with 300 μmol/L H2O2. The H2O2+RSV group was treated with 300 μmol/L H2O2 and 25 μmol/L resveratrol (RSV). The expressions of proteins and genes mentioned above were detected in three groups. NF-κB P65 nuclear translocation was assessed by immunofluorescence technique. (3) HTMCs were divided into 3 groups. The control group was treated with serum-free medium and without TGF-β2 and RSV. The TGF-β2 group was treated with 5 μg/L TGF-β2. The TGF-β2 +RSV group was treated with 5 μg/L TGF-β2 and 25 μmol/L RSV.The expressions of proteins and genes mentioned above were detected in three groups. Results (1) Compared with control group, the protein levels of FN and P-NF-κB P65 were significantly increased in 150, 300, 450 and 800 μmol/L groups,the expression levels of COL1 protein and IL-1β gene were significantly increased in 300, 450 and 800 μmol/L groups (P <0.05). There were no statistical significances between other indicators. (2) The expression levels of FN, COL1, P-NF-κB P65 proteins and IL-1β gene were significantly higher in H2O2 group than those in control group, and which were significantly lower in H2O2+RSV group than th