报道了水稻谷壳硅含量QTLqHUS6的分解研究。针对前期定位的qHUS-6区域,应用在第6染色体短臂RM587-RMl9784区间分离、背景基本纯合的F2:3群体,将qHUS-6分解为2个QTL;其中,qHUS-6-J位于目标区间的上部,qHUS-6—2位于下部。同时,筛选出在目标区间内携带更小杂合片段的3个单株。其中,2株覆盖qHUS-6-J区间,各自交产生了1个F2;3群体,应用这2个群体将qHUS-6-1定位于RM510和RM19417之间约147.0kb的区间内;另1株覆盖qHUS-6~2区间,自交产生了1个F2:3群体,从中挑选出在qHUS-6—2区间具有不同基因型组成的5套F3株系,将qHUS-6—2分解为qHUS-6—2a和qHUS-6—2b,分别位于RM19706-RM19795和RM314-RM19665区间。
The dissection of the QTL qHUS-6 for hull silicon content in rice which was previously located on the short arm of rice chromosome 6 is reported. Using an F2,3 population segregated in the RM587--RM19784 region harboring qHUS-6 in an isogenic background, two QTLs for hull silicon content were detected, of which qHUS-6-1 was located in the upper region and qHUS6-2 in the lower region. Three plants carrying smaller heterozygous segments in the target region were selected, of which two covered the qHUS-6-1 region and the other covered the qHUS-6-2 region. Three F2,3 populations were derived from the selfed seeds of the three plants, respectively. QTL mapping was performed using the two populations segregated in the qHUS-6-1 region, and qHUS-6-1 was delimited to a 147.0 kb region flanked by RMS10 and RM19417. Five groups of F3 lines with different genotypic composition in the qHUS-6-2 region were selected from the other F2:3 population. Two QTLs were separated with two-way ANOVA, of which qHUS-6-2a was located in the interval RM19706--RM19795 and qHUS-6-2b in the interval RM314--RM19665.