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水稻第6染色体短臂谷壳硅含量QTL的分解
  • ISSN号:1001-7216
  • 期刊名称:《中国水稻科学》
  • 时间:0
  • 分类:Q943.2[生物学—植物学] S511.03[农业科学—作物学]
  • 作者机构:[1]中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室,浙江杭州310006
  • 相关基金:基金项目:国家自然科学基金资助项目(30571062);国家863计划资助项目(2006AAl0ZIE8);农业部超级稻专项资助项目(200906).
中文摘要:

报道了水稻谷壳硅含量QTLqHUS6的分解研究。针对前期定位的qHUS-6区域,应用在第6染色体短臂RM587-RMl9784区间分离、背景基本纯合的F2:3群体,将qHUS-6分解为2个QTL;其中,qHUS-6-J位于目标区间的上部,qHUS-6—2位于下部。同时,筛选出在目标区间内携带更小杂合片段的3个单株。其中,2株覆盖qHUS-6-J区间,各自交产生了1个F2;3群体,应用这2个群体将qHUS-6-1定位于RM510和RM19417之间约147.0kb的区间内;另1株覆盖qHUS-6~2区间,自交产生了1个F2:3群体,从中挑选出在qHUS-6—2区间具有不同基因型组成的5套F3株系,将qHUS-6—2分解为qHUS-6—2a和qHUS-6—2b,分别位于RM19706-RM19795和RM314-RM19665区间。

英文摘要:

The dissection of the QTL qHUS-6 for hull silicon content in rice which was previously located on the short arm of rice chromosome 6 is reported. Using an F2,3 population segregated in the RM587--RM19784 region harboring qHUS-6 in an isogenic background, two QTLs for hull silicon content were detected, of which qHUS-6-1 was located in the upper region and qHUS6-2 in the lower region. Three plants carrying smaller heterozygous segments in the target region were selected, of which two covered the qHUS-6-1 region and the other covered the qHUS-6-2 region. Three F2,3 populations were derived from the selfed seeds of the three plants, respectively. QTL mapping was performed using the two populations segregated in the qHUS-6-1 region, and qHUS-6-1 was delimited to a 147.0 kb region flanked by RMS10 and RM19417. Five groups of F3 lines with different genotypic composition in the qHUS-6-2 region were selected from the other F2:3 population. Two QTLs were separated with two-way ANOVA, of which qHUS-6-2a was located in the interval RM19706--RM19795 and qHUS-6-2b in the interval RM314--RM19665.

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期刊信息
  • 《中国水稻科学》
  • 北大核心期刊(2011版)
  • 主管单位:农业部
  • 主办单位:中国水稻研究所
  • 主编:程式华
  • 地址:杭州市体育场路359号中国水稻研究所内
  • 邮编:310006
  • 邮箱:cjrs@263.net
  • 电话:0571-63370278
  • 国际标准刊号:ISSN:1001-7216
  • 国内统一刊号:ISSN:33-1146/S
  • 邮发代号:32-94
  • 获奖情况:
  • 首届全国科技期刊评比二等奖,第二届全国优秀科技期刊评比三等奖,农业部浙江省优秀期刊,国家期刊奖百种重点期刊,百种中国杰出学术期刊,中国精品科技期刊,中国国际影响力优秀学术期刊
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:17826