硅为米饭(Oryza sativa L.) 的最佳的生长是必要的。这研究被进行到好地图 qHUS6.1,为米饭壳硅内容的一个量的特点地点(QTL ) 以前在染色体 6 的短手臂上在间隔 RM510-RM19417 定位了,并且在米饭的不同机关在硅内容上分析这 QTL 的效果。剩余的 Selfed 子孙米饭的异质接合的线在 qHUS6.1 的附近用 13 个 microsatellite 标记被检测。有重叠异质接合的片断的三植物被选择。近的 isogenic 线(无) 的三个集合从 3 植物的 selfed 子孙被开发。他们在一块稻地和硅里被养壳,旗帜叶,和茎的内容在成熟被测量。在一样的无集合在不同 genotypic 组之中基于 phenotypic 分发和变化的分析,重要 genotypic 效果在 NIL 集合被显示出在间隔 RM19410-RM5815 是异质的,而重要效果没在留下被发现, 2 无设置那间隔 RM4923-RM19410 或 RM19417-RM204 在也是异质的。在在 3 个异质的片断的物理位置之中的比较上, qHUS6.1 被 RM19410 限定到 64.2-kb 区域 flanked,包含九的 RM19417 根据 Nipponbare 的染色体顺序注解了基因。三个特点的所有上的显示出的强壮的效果测试了的这 QTL,和提高的等位基因总是从父亲的线 Milyang 46 被导出。现在的学习将便于为 QTL 罚款印射克隆 qHUS6.1 和新基因资源的探索。
Silicon is essential for optimal growth of rice (Oryza sativa L.). This study was conducted to fine map qHUS6.1, a quantitative trait locus (QTL) for rice hull silicon content previously located in the interval RM510-RM19417 on the short arm of chromosome 6, and to analyze the effect of this QTL on the silicon content in different organs of rice. Selfed progenies of a residual heterozygous line of rice were detected using 13 microsatellite markers in the vicinity of qHUS6.1. Three plants with overlapping heterozygous segments were selected. Three sets of near isogenic lines (NILs) were developed from the selfed progenies of the 3 plants. They were grown in a paddy field and the silicon contents of the hull, flag leaf, and stem were measured at maturity. Based on analyses of the phenotypic distribution and variance among different genotypic groups in the same NIL set, a significant genotypic effect was shown in the NIL set that was heterogenous in the interval RM19410-RM5815, whereas a significant effect was not found in the remaining 2 NIL sets that were heterogenous in either of the intervals RM4923-RM19410 or RM19417-RM204. On comparison among the physical positions of the 3 heterogenous segments, qHUS6.1 was delimited to a 64.2-kb region flanked by RM19410 and RM19417 that contains nine annotated genes according to the genome sequence of Nipponbare. This QTL showed strong effects on all of the three traits tested, and the enhancing alleles were always derived from the paternal line Milyang 46. The present study will facilitate the cloning of qHUS6.1 and the exploration of new genetic resources for QTL fine mapping.