中文摘要：

目的：探讨Notch 3受体特异性沉默对急性T淋巴细胞白血病的影响。方法：运用survivin启动子介导的RNA干涉方法特异性地封闭急性T淋巴细胞白血病细胞SupT1中Notch 3基因的表达。结果：survivin启动子介导的RNA干涉系统能特异而高效封闭肿瘤细胞内源Notch 3基因的表达,Notch 3基因表达的下调能够使肿瘤细胞的增殖活性降低,凋亡明显增加。结论：Notch 3基因沉默可有效抑制白血病细胞的增殖,并促进其凋亡,为急性T淋巴细胞白血病相关基因功能研究及靶向性治疗探索了一种新策略。

英文摘要：

Objective：To explore the effects of silencing of Notch 3 on the development of T-cell acute lymphoblastic leukemia.Methods： We investigated the effect of Notch 3 silencing on SupT1 cells using short hairpin RNA（shRNA） driven by survivin promoter.Results： shRNA targeting Notch 3 could down-regulate the expression of Notch 3 effectively.Down-regulating expression of Notch 3 correlated with a significant apoptosis and a proliferation inhibition.Conclusion： Notch silencing by shRNA driven by surviving promoter inhibits the proliferation and promotes the apoptosis of SupT1.This study provides an alternate therapy strategy against Notch signaling pathway.