中文摘要：

为强化三维电极生物膜（3DBER）工艺深度脱氮除磷性能,提髙污水厂尾水质量,将硫磺和海绵铁作为混合填料,构建硫铁复合填料三维电极生物膜（3DBER-S-Fe）脱氮除磷工艺;在不同ρ（C）/ρ（N）、I和水力停留时间（HRT）运行条件下,探究工艺深度脱氮除磷效果.分别从反应器填料和阴极上取生物膜,通过Miseq髙通量测序,构建细菌16SrRNA基因克隆文库.结果表明：在运行条件为ρ（C）/ρ（N）=2、I=150mA和HRT=4h时,3DBER-S-Fe对总氮和总磷的去除率分别可达85.59%和97.43%;适当增加ρ（C）/ρ（N）、I和HRT均能不同程度提髙系统脱氮除磷效率.在填料和阴极上丰度最大的均为具有硫自养反硝化功能的Thiobacillus,分别占40.62%和44.75%;具有氢自养反硝化功能的Rhodocyclaceae在阴极的分布明显多于填料.因此,3DBER-S-Fe具有较髙的脱氮性能主要是硫自养反硝化和氢自养反硝化共同作用的结果,且氢自养反硝化过程主要发生在阴极.

英文摘要：

In order to strengthen the efficiency of the advanced nitrogen and phosphorus removal process of three-dimensional biofilm-electrode reactor（3DBER ） and improve the quality of secondary effluent from wastewater treatment plant, a three-dimensional biofilm-electrode reactor with sulfur/sponge iron mixed fillers （3DBER - S- Fe ） composite denitrification and phosphorus removal process was constructed using sulfur/sponge iron mixed fillers, studies were conducted under different ρ （C ）/ρ（N ）,I and hydraulic retention time （ HRT ） conditions to explore the effects on the performance of advanced removal of nitrogen and phosphorus. Finally,biofilms were taken from the fillers and cathode to build the bacterial 16S rRNA gene clone library by adopting the high-throughput sequencing technologies. Results show that under the condition of ρ （C ）/ρ（N ） =2,I =150 mA and HRT = 4 h,the total nitrogen （ T N ） and total phosphorus （ TP ） removal rate of 3DBER - S-Fe can reach to 85. 5 9 % and 97. 4 3 % respectively. Furthermore,the removal efficiency of nitrogen and phosphorus is strengthened by properly increasing anyone of the three conditions. The largest proportion is Thiobacillus bacteria that can use elemental sulfur as its electron donor which separately accounts for 40. 62 % and 44. 7 5 % of the bacterial community on the fillers and cathode, and the proportion of Rhodocyclaceae bacteria which can use elemental hydrogen as its electron donor reaches more on the latter. Therefore,the result of high nitrogen removal efficiency of the 3DBER - S-Fe mainly depends on the interaction of hydrogen autotrophic denitrification and sulfur autotrophic denitrification, and the process of hydrogen autotrophic denitrification mainly occurrs on the cathode.