中文摘要：

为提高三维电极生物膜工艺脱氮效率,通过运行不同TOC与TN的质量浓度比（ρ（C）/ρ（N））条件下三维电极生物膜-硫自养耦合工艺（3BER-S）,并建立基于反硝化特异性基因nirS克隆文库,研究了ρ（C）/ρ（N）对3BER-S运行特性及反硝化细菌群落的影响.结果表明：ρ（C）/ρ（N）对3BER-S工艺的脱氮效率影响较小,不同ρ（C）/ρ（N）条件下的TN去除效率基本稳定在80%以上.ρ（C）/ρ（N）对3BER-S体系内的反硝化细菌种群结构和营养类型均有一定影响.高ρ（C）/ρ（N）条件下,反硝化细菌种类较少,Thauera（陶厄氏菌属）是体系内的优势菌群,脱氮作用以异养反硝化过程为主;当ρ（C）/ρ（N）降低时,反硝化细菌种类增多,硫自养反硝化细菌所占比例升高.总之,由于硫磺单质的加入,弥补了3BER工艺低ρ（C）/ρ（N）时的反硝化作用电子供体不足,使得3BER-S耦合体系在不同ρ（C）/ρ（N）条件下均能保持高效且稳定的脱氮效果.

英文摘要：

In order to improve the nitrogen removal efficiency of three-dimension-electrode biofilm process,the effects of ρ（C） /ρ（N） on operation characteristics and denitrifying bacteria population community characteristics of a coupled 3-dimensional biofilm-electrode with sulfur autotrophic denitrification technology（3 DBER-S） were investigated at different ρ（C）/ρ（N） conditions.The operating result indicates that there is no significant impact of ρ（C）/ρ（N） on denitrification performance in 3BER-S.And the TN removal efficiency is more than 80%at different ρ（C）/ρ（N） conditions.Moreover,the effects of ρ（C）/ρ（N） on denitrifying bacteria population and denitrification nutrition types in 3BER-S system were found.At high ρ（C）/ρ（N） condition,there is less species of denitrifying bacteria in 3BER-S system,where the Thauera acts as the dominant bacteria and heterotrophic denitrification is the main process.When the ρ（C）/ρ（N） is reduced,both the denitrifying bacteria species and the proportion of sulfur autotrophic denitrification bacteria increase.In a word,the 3BER-S system maintains high and stable nitrogen removal efficiency at different ρ（C）/ρ（N） conditions,because the sulfur makes up for the deficiency of the denitrifying electronic donor in 3 BER-S at low ρ（C）/ρ（N）condition.