中文摘要：

基于电压敏感染料膜电位光学标测的原理，建立了一套光标测系统，主要包括LED激发光源、以及由滤光片、CCD、A/D采集卡及计算机构成的荧光信号采集、处理部分．目前，整套系统已可自动完成图像采集、实时显示，以及部分后处理等工作，已获得兔心室组织细胞膜动作电位．另外，在建立系统的同时，研究了光学标测实验方法，对离体兔心脏急性缺血的主要电生理特征进行了标测实验，并结合仿真研究的结论说明了缺血后有效不应期时空异质性的存在以及组织中电兴奋传导速度的减慢是缺血导致折返性心律失常的主要机制．

英文摘要：

An optical mapping system was developed based on its principle,including the LEDs excitation light,and fluorescence detector consisting of the filter,CCD, A/D sampling card and computer. At present, the system could automatically sample and display the image in real time, and finish some data processing work, The membrance potential of the rabbit ventricle had been successively acquired, In addition, experimental methods of the mapping were studied simultaneously, We mapped electrophysiological effects of the acute global ischemia in the rabbit hearts in vitro, The experiments and our simulation results both suggested that dispersion of refractory period and slow conduction velocity were the fundamental mechanisms of reentry arrhythmia in ischemic myocardium,