玻尔兹曼统计法和均方位移法是两种可用于对非球型生物细胞在简谐光势阱中光阱力的标定方法。用数字实验对这两种标定方法进行了比较,结果表明:与均方位移法相比,玻尔兹曼统计法不仅适用于各向异性非球性细胞,也适用于非简谐、非对称光势阱中任意形状的生物细胞光阱力的标定,结论与已有直接实验相符。
Both Boltzmann distribution method (BDM) and mean square displacement method (MSDM) are available for the force calibration of non-spherical biological cells in a harmonic optical trap. These two calibration methods were compared with numeric experiments. Results indicate that comparing with MSDM, BDM is not only available for the force calibration of nonspherical or anisotropic cells, but also for irregular cells in optical trap potential with inharmonic and asymmetric profiles. The results agree with the experiments reported.