中文摘要：

目的：探讨SLE患者外周血单个核细胞（PBMCs）BLyS基因和蛋白表达及糖皮质激素地塞米松对其表达的影响。方法：梯度密度离心法分离25例SLE患者[（31．40±14．23）岁]和20名女性健康志愿者[（28．20±10．36）岁]的PBMCs，分为2组，地塞米松（1μmol／L）组和培养基组（仅含RPMI一1640培养基），分别于培养0h、6h、12h、24h和72h离心收集PBMCs，用RT—PCR法检测刺激后0至24h时点细胞BLyS mRNA的表达情况，流式细胞仪（FACs）和直接免疫荧光法（激光共聚焦荧光显微镜）检测72h膜结合型BLyS蛋白的表达。结果：（1）SLE患者BLyS mRNA和蛋白表达均高于健康对照（0．40±0．18vs0．27±0．20，P〈0．01；0．37±0．17vs0．25±0．17，P〈0．01；0．42±0．26vs0．29±0．16，P〈0．01；0．39±0．23vs0．24±0．18，P〈0．01）。（2）地塞米松显著抑制健康对照和SLE患者PBMCs BLyS mRNA表达，12h最为明显（0．19±0．10vs0．31±0．16，P〈0．01；0．29±0．18vs0．46±0．21，P〈0．01）。（3）地塞米松显著抑制健康对照和SLE患者PBMCs膜结合型BLyS蛋白表达（2．73±1．89vs3．67±1．64，P〈0．01；3．42±1．53vs4．52±1．95，P〈0．01）。（4）直接免疫荧光法激光共聚焦荧光显微镜检测PBMCs膜结合型BLyS蛋白表达显示地塞米松刺激后BLyS表达减弱。结论：SLE患者PBMCsBLyS表达增强，其表达受地塞米松负影响。

英文摘要：

AIM ： To determine the expression of membrane - bound B lymphocyte stimulator （BLyS） and its mRNA in peripheral blood mononuclear cells （PBMCs） from individuals with systemic lupus erythematosus （SLE）, and to investigate the effect of dexamethasone on BLyS expression. METHODS： PBMCs were obtained from 25 individuals with SLE （ mean age of 31.40 ± 14. 23 ） and 20 female healthy volunteers （ mean age of 28.20 ± 10. 36）. They were randomized into dexamethasone （ 1 μmol/L） group and media group. PBMCs were gathered at 0, 6, 12 and 24 h for BLyS mRNA assessment using reverse transcription - PCR （ RT - PCR）. PBMCs were also collected at 72 h for membrane - bound BLyS protein detection using flow cytometry （FACS） and direct immunofluorescence. RESULTS： （1） The expression of BLyS mRNA and membrane -bound protein were significantly higher in PBMCs from individuals with SLE than that in PBMCs from healthy controls （0.40±0. 18 vs 0.27±0.20, P〈0.01; 0.37 ±0. 17 vs 0.25 ±0. 17, P〈0.01; 0.42 ±0.26 vs 0. 29 ± 0. 16, P 〈 0.01 ; 0. 39 ± 0. 23 vs 0. 24 ± 0. 18, P 〈 0. 01 ）. （ 2 ） Dexamethasone suppressed BLyS mRNA expression in PBMCs from both healthy controls and individuals with SLE, with the greatest effect at 12 h （0. 19 ±0. 10 vs 0. 31 ± 0. 16, P 〈 0. 01 ; 0. 29 ± 0. 18 vs 0. 46 ± 0. 21, P 〈 0. 01 ）. （3） Dexamethasone also inhibited the expression of membrane - bound BLyS protein in PBMCs from both healthy controls and individuals with SLE （ FACs, mean fluorescence intensity, 2.73 ± 1.89 vs 3.67± 1.64, P 〈0. 01 ; 3.42 ± 1.53 vs 4. 52±1.95, P 〈0. 01 ）. （4） Direct immunofluorescence by confocus micrography also indicated that dexamethasone decreased the expression of membrane - bound BLyS protein.CONCLUSION： In vitro, the expression of BLyS mRNA and membrane - bound protein is elevated in individuals with SLE, and dexamethasone has negative effects on its expression.