中文摘要：

DPPA 2 （Developmental Pluripotency-Associated gene 2）是近年来发现的在多能性细胞中特异表达的一个基因，它被认为参与维持干细胞的“干性”．但目前为止，并没有关于该基因在人类胚胎于细胞（human embryonic stem cells，hESCs）分化过程中的表达情况的报道，其功能也尚不清楚．通过Real-time PCR对DPPA2基因在hESCs分化过程中的表达情况进行分析．此外还对其在异常核型hESCs，人类胚胎癌细胞（human embryonic carcinoma cells, hECCs） NTERA-2以及其它5种癌细胞中的表达情况进行检测．结果表明删2基因在hESCs中特异表达，其表达水平随着hESCs的分化而显著下调．该基因在异常核型hESCs和NTERA-2细胞中也有表达．但在其它肿瘤细胞中未检测到该基因的表达．此外，以EGFP-N1系统为基础的亚细胞信号定位结果表明．DPPA2是一个核蛋白．这些结果提示．DPPA2基因可能在维持hESCs特性的过程中发挥着重要的作用．

英文摘要：

DPPA2 （Development Pluripotency-Associated Gene 2） has been identified recently as a gene that is specially expressed in pluripotent cells and considered involving in preservation of sternness status. But the expression file and function of DPPA 2 in human embryonic stem cells （hESCs） have not yet been reported. Here, the expression pattern of DPPA2 was analyzed during hESCs differentiation using real-time PCR. The expression of DPPA2 in abnormal karyotype hESCs, human embryonic carcinoma cells （hECCs,NTREA-2） and other 5 tumor cell lines was also detected. The results showed that DPPA 2 was specifically expressed in hESCs and its mRNA level decreased markedly during hESCs differentiation. DPPA2 was also expressed in abnormal karyotype hESCs and NTREA-2 cells but other tumor ceils. Furthermore, the result of subcellular localization using EGFP-N1 （Enhanced Green Fluorescent Protein） system demonstrated that DPPA2 was a nuclear protein. These data indicated that DPPA2 might be a potential element of the molecular machinery governing crucial properties of hESCs.