中文摘要：

目的：确定上皮-钙黏蛋白（E—cadherin）表达缺失在白血病细胞行为中的作用，最终阐明E—cadherin介导的骨髓微环境与造血细胞相互作用改变在白血病发生中的作用。方法：细胞黏附实验测定HL-60细胞经5-氮杂脱氧胞苷（5-Aza—CdR）诱导E—cadherin表达后细胞黏附能力的改变，MTY法测定细胞增殖能力，细胞迁移实验确定细胞的迁移能力。结果：HL-60细胞经5-Aza—CdR诱导E—cadherin表达后，其对E—cadherin—Fc的黏附能力增加，细胞在E—cadherin—Fc上的增殖能力下降，细胞黏附导致细胞迁移能力下降。结论：白血病细胞中由于E—cadherin表达丧失，引起细胞黏附降低，增殖能力增强，细胞迁移活跃，表明E—cadherin表达缺失与白血病细胞行为的获得有关。

英文摘要：

AIM： To investigate the influence of absent expression of E - cadherin on leukemic cell behavior and further determine the role of abnormal interactions between hematopoietic progenitor and bone marrow on leukogenesis. METHODS ： After E - cadherin was induced with 5 - Aza - CdR in HL - 60 leukemic cells, cell adhesion ability was determined by cell adhesion assay. E - cadherin mediated adhesion associated proliferation was examined by MTF assay, cell mobility was determined by a transwell migration assay. RESULTS ： After E - cadherin was induced by 5 -Aza- CdR in HL- 60 cells, the adhesion ability of cells with E -cadherin -Fc was enhanced. E -cadherin mediated adhesion then inhibited HL -60 cells proliferation and suppressed cell migration ability. CONCLUSION： Absent expression of E - cadherin on leukemic cells plays a role in the decreased adhesion, enhanced proliferation and increased migration ability of leukemic cells. Loss of E - cadherin expression is responsible for the behaviors of leukemia cells.